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Proteomic analysis of Cotesia-associated Host Translation Inhibitory Factors
Upon oviposition, parasitoid wasps inject their eggs along with venom, teratocytes and polydnavirus (PDV) on the host. Among these parasitic factors, PDVs are known to suppress the host immune system and utilize the host translational mechanisms allowing the juvenile parasitoid to develop. Polydnavirusencoded genes can selectively inhibit host translation and still use the translation machinery of the host to synthesize their own proteins. In this study, we utilize a proteomic approach involving two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) that couples isoelectric focusing (IEF) and SDS-PAGE to resolve complex protein mixtures that results from the parastization of Cotesia plutellae on the lepidopteran host, Plutella xylostella. We specifically analyze the changes in protein synthesis using this technique after treatment of HTIFs that has been previously identified on C. plutellae. The difference in protein profile due to parasitization was confirmed by in vitro translation assay using rabbit reticulosyte lysate.
