한국응용곤충학회 학술대회논문집

이 간행물 논문 검색


2009년도 한국응용곤충학회 춘계학술발표회 (2009년 5월) 209

2009.05 구독 인증기관 무료, 개인회원 유료
Historically in Japan, studies on the diseases of the silkworm, Bombyx mori, as a factor affecting the well-being of the silk industry, have dominated insect pathology. However, work by Hidaka (1933) demonstrated the possibility of controlling the pine moth, Dendrolimus spectabilis, with the fungus Beauveria bassianaand since then, various attempts have been made to develop a method to control insect pests using insect pathogens (Table 1, 2). The cypovirus product, Matsukemin, was the first microbial control product to be registered in 1974, and inactive and live Bacillus thuringiensis products were also registered and put on the market as pesticides in 1980 and 1981, respectively (Table 3). Currently, there are 32 microbial insecticides on the market that constitute slightly less than 2% of all insecticides used in Japan (Table 4, Fig.1, 2). Adoption of biopesticides is likely to increase in the near future due to scientific advances and several new government policies that encourage the use of alternative pest control products.
2009.05 구독 인증기관 무료, 개인회원 유료
Swallowtail butterfly’s (Papilionidae) family is a large group including at least 550 species in the world, one of the most evolved butterfly families, and selects many special plants as a host plant. Many scientists have studied on the mechanism for host selection by swallowtail butterflies and many results including attractant, ovipositional stimulant, and feeding stimulant in each host plant have been reported. However, most of the reports are related to Papilinonini and Troidini genus and not to Graphiini genus. The reason why there are few reports about this genus is based on their nature. That is, this genus butterflies, especially common bluebottle, Graphium sarpedon, are very nerves and rearing for successive generations is difficult in the laboratory, emergence-adult never laid their eggs on the host plant and so on. We, therefore, have tried to isolation and identification of attractant, feeding stimulant, and ovipositional stimulant in the camphor tree to the common bluebottle, G. sarpedon.
2009.05 구독 인증기관·개인회원 무료
돌발하여 큰 피해를 주었다. 벼 줄무늬잎마름병은 1935년부터 몇 년간의 주기로 계속 대발생하여 1937년 낙동강 연안에, 1940년 충남, 경북을 비롯한 경남 밀양, 진영, 창원, 진주, 전남 구례에, 1965년에는 전국적으로 대발생하여 6.5%의 피해를, 1973년에는 전국적으로 발생하여 6%의 피해를 주었다 (Chung, 1974). ‘70년대에는 다수계 품종이 보급되어 병발생이 잠시 추춤하였 으나 ’80년대에 일반계품종이 다시 재배되면서 1984년을 제외하고는 ‘90년대 말까지는 대발생의 피해는 없었다. 그러나 2001년 경기 김포, 강화지역과 2007년에 전북 부안, 충남 서천지역에 국지적으로 대발생하여 국지적으로 대 발생하여 큰 피해를 주었다. 그동안의 애멸구에 대한 연구결과를 분석하여 2000년대 이후 간헐적인 대발생 원인과 줄무늬잎마름병의 발병을 줄일 수 있 는 방안을 위한 연구방향에 대해 몇가지 제안을 한다. 기존의 연구에서 줄무늬잎마름병의 발병 과정은 자세히 연구되었으며 병의 대발생과 관련된 원인으로 애멸구의 보독충율과 애멸구의 밀도를 중요시하고 있다. 이제까지의 연구에서 병의 발병과 전염에 중점을 둔 연구에서는 바이러 스의 특성과 전염원인 기주범위, 매개충의 보독충율에 초점이 맞추어져 왔으 며, 충의 연구에서는 병의 발생과의 연관성 보다는 애멸구의 생태에 대한 연 구에 초점이 맞추어져 병의 발생과 관련된 상호작용에 대한 연구는 대단히 미흡한 실정이다. 애멸구의 경우 월동중의 기온이 높으면 월동율이 높아지나 이것이 꼭 벼의 줄무늬잎마름병 대발생의 원인이 되는지, 줄무늬잎마름병이 대발생할 때는 꼭 보독충율이 높아야 하는지, 보독충율이 높으면 줄무늬잎마 름병이 대발생을 하는 것인지, 월동후 증식과정에서의 대발생은 어떻게 일어 나는지, 과거에는 남쪽에 발생이 많았는데 왜 서해안지역에 많아지는지, 등 앞으로 해결해야 될 과제가 많이 있다. 이러한 의문들을 해결하기 위해서는 장기적인 모니터링 성적이 필요하다. 이번 줄무늬잎마름병 발생으로 과거 성 적을 조사해보니 연속해서 계속적으로 조사된 성적이 별로 없었다. 문제가 될 때마다 반짝하고 2∼3년간 조사하다 과제가 끝나면 잊어버리고, 다시 문제가 발생할 때 다시 시작하는 형태의 연구가 되었다. 인원이 적고 할 일은 많아 계속적으로 조사하기가 어려운 현실이지만 지속적인 연구가 이루어져야 위 의문들이 풀릴 것이며 항구적인 대책이 마련될 것이다.
2009.05 구독 인증기관·개인회원 무료
Chemical ecology has been useful in various aspects of biosecurity that requires dynamic multidisciplinary practices. Some techniques and research outcomes have already been incorporated as routine practices in biosecurity, while many others are yet to come. Many pheromones and other semiochemicals have been being used as highly sensitive, species-specific detection and surveillance tools. When different species interact each other, specific volatile chemicals are often produced. Detection of these specific signature volatile compounds can also be used as an early diagnostic tool. Highly sensitive and discriminating olfactory tissues can be used as low-level chemical sensors. Risk analysis is an important component of biosecurity program. The range of host plants may be predictable by using information on ORN response profiles of a given species and on volatile chemical profiles of the candidate plants. In a study using two Cydia species as a model system, we hypothesize that combinational input from two different groups of olfactory receptor neurons (ORNs), antagonist ORNs and agonist ORNs, may play a key role in discriminating host plants from non-host plants. Semiochemicals can be used as delimitation and eradication tools against invading species. For example, mass trapping with pheromone traps, combined with some other means such as sterile insect technique, effectively eradicated the painted apple moth, an invasive species, in New Zealand. A cooperative research program among New Zealand, USA and Australia is currently underway to use semiochemicals to eradicate the light brown apple moth invading California. Chemical ecology in aquatic environment has been poorly understood. Semiochemicals for marine organisms would also be useful to improve marine biosecurity.
2009.05 구독 인증기관·개인회원 무료
The Wolbachia bacterium belongs to α-proteobacteria and is one of the most prevalent intracellular endosymbionts of arthropods and nematodes. One theoretical study suggests that the bacterium may infect up to 66% of insect species. Its fundamental biology and potential applications have been paid much attention with its ability to induce 4 distinct reproductive anomalies and other modifications of its various hosts. The four reproductive anomalies include, i) cytoplasmic incompatibility, where embryonic death results from the cross between an uninfected female and an infected male, ii) feminization, where an infected egg with male genetic background develops into an infected functional female, iii) male killing, where infected eggs with male genetic background dies in their early developmental stage, iv) parthenogenesis, exclusively where, in organisms with haplodiploid sex determination, an infected unfertilized egg develops into an infected female. All these reproductive anomalies, except cytoplasmic incompatibility, cause female biased sex ratio because females can descend cytoplasm to the next generation where the bacterium can reside. Furthermore, these have nothing to do with hosts’ evolutionary interests but only with the bacterial evolutionary interests (i.e. its prosperity). In this sense, the bacterium is one of the pronounced examples of selfish genetic elements. Recent researches also focus on some novel phenomena other than these reproductive anomalies mentioned above. We will review recent advances in general biology of the bacterium, its impacts on host life history, the reproductive anomalies and cellular mechanisms underlying them and potential utilization of the bacterium for better agricultural and medical environments.
2009.05 구독 인증기관·개인회원 무료
Six species of the Pristocerinae are recognized from Korea, including two new species (Pseudisobrachium sp. nov., Parascleroderma sp. nov.), and four species new to Korea (Acrepyris minutus (Yasumatsu 1955), Apenesia elegans Terayama 1999, A. okinawensis Terayama 1999, and Pristocera formosana Miwa et Sonan 1935). The illustrations of diagnostic characters are presented with a key to species of Korean Pristocerinae is provided.
2009.05 구독 인증기관·개인회원 무료
The bumblebee, Bombus ardens, is a valuable natural resource, and is most notably utilized for greenhouse pollination. In order to gain a greater understanding of the population genetic structure and the genetic diversity of this species, we sequenced a portion of the mitochondrial COI gene corresponding to the “DNA Barcode” region (658 bp) from 160 individuals collected over 15 Korean localities. Uncorrected pairwise distances among the eight haplotypes suggested low intraspecific genetic diversity, with a maximum sequence divergence of 0.3%. Such a low level of intraspecific genetic diversity was further reflected in local populations, particularly to islet populations, such as those of Youngheungdo, Jakyakdo, and Ulleugdo, which had zero genetic diversity. Geographically, one haplotype (BARBA01) was widespread and dominant, with a frequency of 90.6% (145 among 160 individuals). Other haplotypes were restricted to one to three localities and had low frequency. Overall, a very high rate of per generation female migration ratio (Nm= 4.6 to infinite) and a very low level of genetic fixation (FST= 0 to 0.099) were detected between pairs of localities, suggesting that the B. ardens population sonthe Korean peninsula are panmictic, which is consistent with our understanding of their dispersal capability.
2009.05 구독 인증기관·개인회원 무료
Invertebrate mitochondrial genome contains 13 protein-coding genes and major start codons for them are ATA (Met) and ATG (Met). However, alternative start codons such as ATT (Ile), ATC (Ile), TTG (Leu), and GTG (Val) also have been suggested from a diverse organism. Approximately 120 complete mitochondrial genome reported showed that the start codon for COI gene evidences an array of diverse designation of COI start codon such as typical ATN, tetranucleotide TTAG and ATAA, newly proposed AAT and AAC and so on. In the case of Lepidoptera, many completely sequenced species showed no typical start codon at the start context of COI and even within the neighboring tRNATyr. In order to clarify, we newly sequenced the beginning context of COI gene, encompassing the neighboring tRNATyr and start region of COI gene from 39 species belonging to eight lepidopteran families. We found the newly sequenced 39 species and 14 available complete lepidopteran mitochondrial genomes all possessed CGA (arginine), which is the first non-overlapping in-frame codon in COI gene. Furthermore, this CGA is highly well aligned in terms of both nucleotide and amin o acid sequences with neighboring region. Thus, the CGA (arginine) may be synapomorphic character for Lepidoptera, functionally constrained. We, therefore, propose the CGA sequence as the start codon for COI gene in lepidopteran insects.
2009.05 구독 인증기관·개인회원 무료
The 15,389-bp long complete mitogenome of the endangered red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, which have the gene order of tRNAMet, tRNAIle, and tRNAGln at the beginning. Due to the uncertainty the start codon for COI gene in insect has been discussed extensively. We propose the CGA sequence as the start codon for COI gene in lepidopteran insects, based on complete mitogenome sequences of lepidopteran insects including our P. bremerii and additional sequences of the COI start region from a diverse taxonomic range of lepidopteran species (a total of 51 species belonging to 15 families). As has been suggested in other sequenced lepidopteran insects the 18 bp-long poly-T stretch and the downstream conserved motif ATAGA that were previously suggested to serve as a structural signal for minor-strand mtDNA replication also was found at the 3’-end region of the P. bremerii A+T-rich region. In an extensive search to find out tRNA-like structure in the A+T-rich region, each one tRNATrp-like sequence and tRNALeu (UUR)-like sequence were found in the P. bremeri A+T-rich region, and most of other sequenced lepidopteran insects were shown to have tRNA-like structure within the A+T-rich region, thereby indicating that such feature is frequent in the lepidopteran A+T-rich region. Phylogenetic analysis using the concatenated 13 amino acid sequences and nucleotide sequences of PCGs of the four macrolepidopteran suferfamilies together with Tortricoidea and Pyraloidea well recovered a monophyly of Papilionoidea and a monophyly of Bombycoidea. However, Geometroidea and Noctuoidea were unexpectedly clustered as one group and placed this group to the sister group to Bombycoidea, instead of Papilionoidea in most analyses.
2009.05 구독 인증기관·개인회원 무료
The IMGD (Insect Mitochondrial Genome Database; http://www.imgd.org) archives 113,985 partially sequenced hexapod mitochondrial genome entries (PMEs), providing various information such as the number of mitochondrial genes or taxonomic information. The 113,985 PMEs show most PCGs including much larger number of PMEs than rRNAs and tRNAs, and the family, genus, and species information are focused on five orders, Coleoptera, Diptera, Lepidoptera, Hymenoptera, and Hemiptera in 31 hexapoda orders. The alignment of 58,238 COI gene entries shows discordance in fragment length and portion, resulting DNA barcode using 5′-region cannot be applied for all COI gene entries overall hexapod orders.
2009.05 구독 인증기관·개인회원 무료
Camptomyia is a genus of gall midges belonging to subfamily Porricondilinae. On the study of shiitake mushroom farm in Korea, three species of maggots were collected. Among them, two Camptomyia spp. were recognized as the major pests. Colonies of these maggots were feeding on the mycelium of shiitake mushroom, disturbing the formation of mushroom fruit body. The morphology of C. heterobia and C. corticalis were illustrated with the description.
2009.05 구독 인증기관·개인회원 무료
The soybean aphid, Aphis glycines Matsumura 1917, is well known as a soybean pest in the world. Recently, it has been introduced to North America causing serious damage in U.S. As a cooperative research with USDA-ARS, we have investigated A. glycines in soybean fields, and also examined the colonies on the overwintering host Rhamnus davurica in order to find its natural enemies. It was generally reported that A. glycines has host alternation between the soybean, Glycine max (summer host) and the Dahurian buckthorn, Rhamnus davurica (winter host) in East Asia. However, it was very difficult to identify the soybean aphid, A. glycines, from R. davurica due to the co-existance of at least three Aphis species and the seasonal polymorphisms of each species (e.g, gynopara, ovipara, and male). For species identification, we tested 3 molecular markers, mitochondrial COI, COII, and nuclear EF1α, for 14 collected samples (7 samples from G. max and 7 samples from R. davurica). As a result, we found two different species, A. gossypii and other Aphis sp., are mixed together with A. glycines on R. davurica. We report the biology of A. glycines in Korea, and present species identification using molecular phylogenetic approach.
2009.05 구독 인증기관·개인회원 무료
On the study of phyline plant bug, four species of the genus Psallus Fieber have been observed as new record to Korea. According to the Korean Catalog (Yong Jung Kwon, Sang Jae Suh and Jung Ae Kim, 2001) nineteen species are recorded from the Korean peninsula. Our recent investigation discovered four additional species, Psallus cinnabarinus Kerzhner 1979, P. flavescens Kerzhner 1988, P. stackerlbergi Kerzhner 1988 and P. loginovae Kerzhner 1988. All the four species are described with genital illustration, biology and host information.
2009.05 구독 인증기관·개인회원 무료
DNA barcode is known to be successfully applied in identification on the members of Insecta. In recent studies, however, it was known that the DNA approach may fail in several taxa with following cases: (1) very recent speciation and hybridization, (2) recent diverged groups with complex gene histories, (3) the spread of maternally transmitted bacteria, (4) adding more than one geographical race and at least one congener, (5) different levels of dispersal. In this study, we taxonomically review on the Korean Hatchiana using the morphological data and DNA barcodes. In morphology, they are distinct from each other by the characteristics of body coloration, eye, pronotum, scutellum, and aedeagus. In molecular data, however, the interspecific sequence distance ranged from 0.0-3.4%. This result is caused by H. glochidiatus, of which the sequence divergence is 0.2-2.8% in H. rosinae, 0.8-2.6% in H. baekripoensis, and 0.0-3.3% in H. jirisanensis. Also, H. glochidiatus produces mixed-clusters with H. rosinae and H. jirisanensis in NJ phenogram. Through this presentation, therefore, we discuss on why the four Korean Hatchiana species distinct by morphological characters produce mixed-clusters in DNA barcoding.
2009.05 구독 인증기관·개인회원 무료
The flower bugs (Heteroptera: Anthocoridae) is usually known as biological control agents against various kinds of agricultural pests such as aphids, mites, thrips and so on. Since the classification of the family Anthocoridae has been controversial, the molecular phylogenetic study was conducted with 44 species including 6 outgroup taxa. Three genes, a total of 3277 bp of sequence data (nuclear 18S rDNA: 2022bp, 28S rDNA: 755bp, and mitochondrial 16S rDNA: 498bp), were analyzed using ML (maximum likelihood) and Bayesian methods, excluding MP (maximum parsimony) as the incongruence length difference (ILD) test has very low (0.001) P-values on all partition tests. Our results support the rank and monophyly of the family Lasiochilidae which was exclusively separated from the main clade of Anthocoridae proposed by Schuh and Stys (1991), and indicate the monophyletic relationships among tribes and genera within the family Anthocoridae. According to our results, the genus Amphiareus should be out of the tribe Dufourini and treated as the tribe level and The three genera, Blaptostethus, Scoloposcelis and Xylocoris should be placed to the family Lyctocoridae. We also propose the evolutionary theory of Anthocoridae based on their habitats, hunting behavior and the molecular phylogenetic results.
2009.05 구독 인증기관·개인회원 무료
The root zone applications of a systemic insecticide, carbofuran, were evaluated for their impacts on the brown planthopper, Nilaparvata lugens (Stål), and spider populations in the greenhouse and rice paddy fields. In the green house experiments, no BPH nymphs were hatched at root zone treated on 40 to 50 day-old rice, while around 20 to 54 nymphs per pot were emerged in broadcasting and foliar spray treatments. This indicates that the root zone treatment can kill the eggs of BPH effectively. This is the first study ever demonstrated the high egg mortality of BPH due to the root-zone application. In the field experiments, the density of BPH in root zone treated plots were four to six times lower than in broadcasting and foliar spray plots at the 21 days after application. The BPH outbreaks and hopper-burns were observed at all treatments except the root zone treated plot at the 28 days after application. The root-zone application did not impact on the spider population, while foliar spray killed most of all spiders just one day after application. The densities of spider in foliar spray plots were always lower than in root-zone treated and control plots. The results indicated that the root-zone application of carbofuran can control BPH effectively without adverse effects to the spiders inhabited on the paddy field.
2009.05 구독 인증기관·개인회원 무료
지역적으로 멀리 떨어져서 서로 유전적 유상성이 멀 것으로 생각되는 두 계통 제주계통과 횡계 계통의 배추좀나방 개체별 아세틸콜린에스테라제 1과 2의 유전자 염기서열을 비교하여, 유전자 염기서열 내에 염기의 다양성이 존 재하는 곳이 몇몇 확인되었다. 위의 염기서열을 바탕으로 염색체의 유전자 변 이도를 그려본 결과, Ace1 유전자의 변이가 더 높아 지역 계통 간 변이율을 비교할 때 Ace1 유전자가 지역 계통 군집 분류 마커로 적당할 것으로 판단되 어 유전자 마커로 선발하였다. Ace1 유전자 변이 분석 결과를 바탕으로 지역 계통 군집분석을 실시한 결과, 횡성과 둔내 그룹, 태백, 임계, 횡계 등 고령지 배추생산단지로 한 그룹, 홍천, 평창과 제주도의 안덕 등이 군집 분석에서 그 룹으로 분류되어 생물검정 결과로 군집 분석한 것과 같은 경향을 보이고 있 다. 이는 배추좀나방이 지역성 특성을 갖고 있음을 말해주는 것으로 지역 월 동의 가능성을 시사하고 있다. 이를 바탕으로 배추좀나방 관리는 중부내륙지 역(홍천, 횡성, 둔내), 고령지 지역(평창, 임계, 횡계, 태백), 덕유산지역(무주), 제주지역(안덕, 성산)으로 나누어 관리하는 것이 효율적이라 생각된다.
2009.05 구독 인증기관·개인회원 무료
보푸란에 대한 배설량 저해 반응을 토대로 약제 처리 방법 별로 실제 벼멸구 한 마리를 죽이는데 필요한 약량을 구하여 이미다클로프리드와 카보푸란의 처리 방법에 대한 처리 방법 별 실제 살충 효과 양을 조사하여 비교한 결과, 이미다클로프리드는 약제를 뿌리에 처리, 약제 침투에 의한 살충 효과를 나타 내는 처리 방법이 효과가 가장 좋았다. 다음은 잎 침지처리에 의한 접촉 효과 가 좋았으나 감수성에서는 미량국소 처리에 의한 살충 효과 발현에 필요한 양과 별로 차이가 없었다. 카보푸란은 미량국소처리의 약제 직접 접촉 방법이 상대적으로 효과가 가장 좋았다. 처리 방법 별 약제 필요량을 뿌리 처리와 잎 침지 처리에서 시간 별 조사한 결과는 이미다클로프리드의 약제 처리 효과는 처리 약 4일 후에, 카보푸란은 처리 약 2일 후 약효가 충분히 발현됨을 보여 주었다. 벼멸구 개체 살충 필요 약량에 의한 저항성 비는 이미다클로프리드 의 경우 뿌리처리와 잎 침지처리에서 약 50, 미량국소처리에서 약 80을, 카보 푸란의 경우 각각 약 4-6배를 나나냈다
2009.05 구독 인증기관·개인회원 무료
과류의 검역해충에 사용되는 methyl bromide(MB)를 대체할 수 있는 후보 물질이다. 우리나라의 수출용 단감 수확과에 잔존하는 해충을 박멸하기 위한 MB 대체용 훈증 제를 개발하기 위하여 단감 잔존 해충에 대한 EF의 훈증효과를 검토하였다. 25±1℃ 에서 훈증용 desiccator에 여름형 점박이응애(Tetranychus urticae)를 넣고 EF 18.7, 24.7 mg/L(vapormate 112, 148 mg/L)의 농도로 6시간 처리하였다. 처리 후 한 시간 동안 환기시킨 다음 25±1℃ 16L:8D의 조건에서 보관하며 24, 48시간 후 살비율을 조사하 였는데 24시간 후 두 처리에서 모두 100%의 살비율을 기록하였다. 또한 15±1℃에서 desiccator에 단감을 0, 30% 채우고, 점박이응애의 알과 월동형 성충에 대하여 EF 16.9, 23.3, 35.3 mg/L(vapormate 101, 140, 212 mg/L)의 농도로 6시간 처리하였다. 처 리 후 한 시간 동안 환기시킨 다음 점박이응애의 알은 25±1℃ 16L:8D의 조건에서 보 관하며 5일 후 부화율을 조사하였고, 월동형 성충은 동일 조건에 보관하면서 24, 48 시간 후에 살비율을 조사하였다. 실험에 사용된 단감은 1±1℃에 보관하며 7, 14일 후 에 약해를 조사하였고, 처리 15일 후 단감 꼭지 속에 있는 잔존해충들의 살충율을 조사하였다. 점박이응애의 알은 모든 처리에서 100% 살란되었다. 월동형 점박이응애 성충에 대한 살비율은 30%의 용적율, EF 16.9, 23.3, 35.3 mg/L(vapormate 101, 140, 212 mg/L)처리에서 각 12.2, 26.7 46.7%이었다. EF를 처리한 단감에서 약해는 발생하 지 않았다. 처리된 단감 꼭지에서는 감나무주머니깍지벌레, 점박이응애, 톡토기 등이 발 견되었으며 EF 16.9 mg/L(vapormate 101 mg/L)처리를 제외한 모든 처리에서 100%의 살 충율을 나타내었다. 색도, 반점, 무름 등의 약해를 처리 후 5주 동안 일주일 간격으 로 조사하였고, 처리 5주 후에는 경도를 조사하였다. 모든 약해조사에서 약해는 없는 것으로 조사되었다. 이상의 결과로 보아 EF는 월동형 점박이응애에 대해서는 방제효 과가 떨어지지만 점박이응애 알과 여름형 성충 및 기타 단감 잔존 해충에 대해서는 살비 및 살충효과가 우수한 것으로 나타났다. 앞으로 EF를 실용화하기 위한 연구가 더 이루어져야 할 것으로 생각된다.
2009.05 구독 인증기관·개인회원 무료
연구는 엽채류 재배지에 많은 피해를 주고 있는 파밤나방(Spodoptera exigua) 에 대하여 신규 살충제인 Bistrifluron 10% EC와 혼합 살충제 Bistrifluron+Chlorfenapyr 13% SC의 발육 단계별 살충활성, 속효성 및 잔효력을 확인하고자 수행되었다. Bistrifluron 10% EC의 발육단계별 시험에서 알에 대한 부화억제효과는 77.9%이었으며, 부화 후 생육 단계에도 영향을 주어 98.3%의 우수한 살충효 과를 나타내었다. 파밤나방 2령 유충에 대한 LC50과 LC90은 각각 1.33ppm과 12.52ppm이었으며, 잔효력은 10일까지 지속되어 IGR계 살충제중 가장 우수한 살충효과를 나타내었다. IGR계 살충제의 부족한 속효성과 대상해충의 확대를 위한 혼합제로 Bistrifluron+ Chlorfenapyr 13% SC를 선정하였으며, 그 결과 알에 대한 부화억제효과는 95%이었으며, 2령과 4령 유충에 대하여 각각 100%의 방제효과를 나타내었다. 파밤나방에 대한 속효적인 효과는 24시간 이내에 4령 유충을 100%로 방제가 가능하였으며, 그 효과는 14일까지 지속되었다. Bistrifluron 10% EC와 Bistrifuron+Chlorfenapyr 13% SC는 파밤나방에 대한 부화억제, 속효성 및 잔효력이 우수하여, 파밤나방의 방제에 대한 활용성이 높을 것으로 판단된다.
1 2 3 4 5