The first list of the family Tortricidae from North Korea was provided by Zhu(1969), including 16 species of the subfamily Olethreutinae. There had been no research on this group, until the report of Park and Byun (1991) with 9 additional species. And Jaros et al.(1992) reported 17 previously unrecorded species, including 4 of Eucosmini. Byun et al. (1998) made a illustrated catalogue of Korean Tortricidae containing 350 species. In 1999, Razowski provided a list of 358 tortricid species in the Korean Peninsula, including 87 species of the subfamily Olethreutinae from North Korea. Among them 29 species of the tribe Eucosmini were included as them to be distributed in North Korea.
In this study, we report eight previously unrecorded species of the tribe Eucosmini from North Korea, based on the examination of the material deposited in the Hungarian Natural History Museum.
The species, which are known for the first time from North Korea in this study, are as follows.
1. Ancylis melanostigma Kuznetsov
2. Ancylis sativa Liu
3. Eucoenogenes japonica Kawabe
4. Eucosma glebana (Snellen)
5. Notocelia rosaecolana (Doubleday)
6. Rhopobota ustomaculana (Curtis)
7. Spilonota ocellana (Denis et Schiffermüller)
8. Zeiraphera lariciana Kawabe
The family Gracillariidae belonging to Gracillarioidea comprises more than 2,000 described species of 75 genera in the world (Davis & Robinson, 1999). The members of the family are mostly small-sized moths, which can be distinguished by a short (2-10mm in length) and lanceolated narrow wing with prominent cilia. The larvae of the family have been known as leaf-miner and hypermetamorphic development in larval stages.
In Korea, it has been recorded 39 species of 11 genera by several researchers (Nakayama & Okamoto, 1940; Ko, 1969; Park, 1975; Park et al. 1977; Kumata & Park, 1978; Park, 1983; Kumata et al., 1983; Park & Han, 1986). Recently, Sohn (2007) reported 6 species without descriptions and detail information for the species. However, it is necessary to investigate and review the Korean Gracillarid moths, due to their lack knowledge from Korea to date, comparing with Japanese fauna, which contains 225 known species from Japan (http://www. jpmoth.org/).
In this study, three species, Cloptilia heringi Kuroko, Phyllonryeter cavella (Zeller), and P. tritorrhecta (Meyrick), of the family Gracillariidae aree reported for the first time from Korea.
Genus Hellinsia Tutt, 1905 is the largest group of the tribe Oidaematophorini (Lepidoptera, Pterophoridae, Platyptilinae). The genus is mostly distributed in the Neotropical region and Nearctic region, and partly in the Palaearctic and Afrotripical regions, with more than 190 described species from all over the world (Gielis, 2003). Among them, approximately 30 species of the genus Hellinsia Tutt have been reported in the Palaeartic region, including 9 species of Japan, 14 of Russia, and 15 of China. However, it has been poorly known from Korea to date, with only 4 known species: 2 species (H. lienigianus (Zeller) and H. distinctus (Herrich-Schffer) from South Korea (Park 1993, 1994) and 2 species (Hellinsia albidactylus (Yano) and H. lateolus (Yano)) from North Korea (Arenberger, 1991).
This study is aimed to review all known species of the genus Helinsia Tutt in the Korean Peninsula, with three previously unrecorded species: Hellinsia ishiyamanus (Matsumura), H. Kuwayamai (Matsumura), and H. nigridactyla (Yano). Material examined in this study are preserved in the Insect collection, Korea National Arboretum, Korea. Consequently, 7 species, in total, are recorded from the Korean Peninsula.
A new species of Adoxophyes is described, illustrated, and distinguished from closely resembling species. Larvae of this species were discovered on sweet cherry (Prunus avium) imported from China and intercepted under plant quarantine inspection at Korean sea- and airport. They were reared to adult in the laboratory for identification. We provide a full description of the new species based on adult of female external structures. In addition, we conducted a comparative study of three related species (A. orana, A. honmai, and A. new species) and provided a key to distinguish them.
To confirm the identity of new species, we compared barcoding sequences (~472 bp) of the mitochondrial cytochrome c oxidase subunit I (COI) gene from 11 specimens of three Adoxophyes species. As a result of this study, we confirmed the identity of Adoxophyes new species. We also provided pairwise p-distances among the species and their neighbor-joining tree.
The genus Cydia Hübner is distributed in all the continents, with the tropical regions. However, It's rather insufficiently studied. In palaearctic, there are about 100 known species, less than half of this amount is Nearctic region; 49 species are recorded from Europe. The repartition areas of some species are wide, of some other small, however, they extend during the studies.
The genus Laspeyresia Hübner, 1825, which was often used instead of Cydia until the late 1970s is help to be a junior homonym of Laspeyresia R.L,. 1817, an emendation of Laspeyria Germar, 1810 (Noctuidae). Comprehensive reviews of Cydia have been given by Heinrich (1926), Obraztsov (1959), Danilevsky and Kuznetzov (1968), and Razowski (1989). Komai (1999) discussed the phylogenetic position within the tribe (Horak, 2006).
In the present study, the Korean species of genus Cydia are reviewed. A total of 10 species are recogniged. Of them, Cydia danilevskyi (Kuznetzov, 1973) and Cydia indivisa (Dailevsky, 1963) are reported for the first time from Korea. Morphological characters of this genus are redescribed with illustrations of adults, wing venation, male and female genitalia. Distribution map, data matrix, cladistic analysis are provided.
Multivariate morphomatric analyses were conducted to cluster the morphologically similar group using species units within the tribe Aphidini. Some species of the genus Aphis are morphologically very similar each other and, mentioned by aphid taxonomists as well, possibly grouped by some characteristics. To cluster the morphologically related groups and find some significant characteristics to define morphological groups for 59 Aphidini species, we perform two statistical analyses of 30 morphomatric characters, Principle Component Analysis (PCA; SAS Procedure PRINCOMP) and Canonical Discriminant Analysis (CDA; SAS Procedure CANDISC) using SAS/STAT version 9.1.3 (SAS Institute, Inc., Cary, North Carolina). The results of the morphological clustering analyses were compared with the molecular phylogeny of Aphidini obtained from the previous study using three molecular gene fragments: partial mitochondrial tRNA-leucine + cytochrome oxidase II (tRNA/COII), partial mitochondrial 12S + tRNA-valine + 16S (12S/16S), and nuclear elongation factor-1 alpha (EF1α) (total 3,289bp) for 37 representative species among them. The congruence or difference between morphological and molecular analyses is also discussed in Aphidini group
Since Miyamoto & Lee (1966) first recorded three species of Miridae, many species including new species are added to the fauna of Korea by several Korean and foreign taxonomists (Josifov, 1992; Josifov & Kerzhner, 1972; Lee & Kwon, 1991; Lee et al., 1993; Lee et al., 1994). Kwon et al. (2001) collectively arranged the Korean fauna of Miridae into 7 subfamilies, 84 genera, and 209 species, but many species have been believed to remain unrecorded. During recent the recent study of Miridae, we found three unrecorded species, Apolygus roseofemoralis (Yasunaga 1992), Pachylygus nigrescens (Kerzhner 1977), Deraeocoris yasunagai Nakatani 1995. The brief information on them are presented with the photos of specimens. All specimens are deposited in NIAST.
Camptomyia is a genus of gall midges belonging to subfamily Porricondilinae, with 42 species known from the palaearctic region.
In November 2007, serious damages of the Shiitake mushroom logs were reported in many shiitake farms from the middle part of South Korea. Rearing the adults in laboratory, a Camptomyia species was recognized as the major species causing damages on shiitake. Colonies of maggots were observed, feeding the mycelium of shiitake mushroom and the phloem of oak timbers, disturbing the mushroom fruit body formation.
The morphology of the larvae and adult of this Camptomyia heterobia. is illustrated with the description of the damage symptom.
Odontepyris sp. nov., a new ectoparasitoid on the caterpillar of Telorta divergens (Butler, 1879) (Lepidoptera: Noctuidae), is recognized from Korea. After rearing the larvae on the host insect, adult females were obtained and described. Revising all known species of genus Odontepyris, a key to the species of the genus Odontepyris is presented for the Eastern Palearctic region.
Sclerodermus harmandi populations were collected from 6 Korean regions (Seoul, Gangwon, Chungcheong, Jeolla, Gyeonasang and Jeju provinces), 1 Chinese region (Nanjing) and 1 Japanese region (Tokyo). Above all was identified as the same species based on morphological characteristics. In present study, we compared the intra-specific characteristics of regional isolated populations of S. harmandi based on nucleotide and amino acid sequences of variant genomic and mitochondrial loci: the full length of internal transcribed spacer 1 and 2 (ITS1 & ITS2), the complete cytochrome oxidase subunit I and II (COI & COII) and tRNA genes for aspartate and lysine and 5'end of ATPase 8 genes. There were no significant differences among the analysed 9 populations regardless of regional segregation. The nucleotide and translated amino acid sequences similarities were significantly high, despite some sequence differences were found in same loci. This result support the report based on the morphological characteristics. However, we are conducting further study to identify whether sequence differences in specific loci are the characteristics of individual level in population or population level and to analyse the full sequences of mitochondrial DNA by regional segregation.
The completely sequenced mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys, is a circular molecule of 16,518 bp with a total A+T content of 76.4%. Nucleotide composition and codon usage of this genome are near the means observed in other 12 hemipteran mitochondrial genomes; however, the initiation codon for CO1 gene appears to be TTG, dissimilar to what has been seen in the 12 mitochondrial genomes. In this genome, the A+T rich region between srRNA and tRNAIle gene includes two extensive repeat regions, in which each region includes 4 and 12 tandem repeats of a 73 bp sequence, respectively. The gene content, order, and structure of the H. halys mitochondrial genome are consistent with that of Triatoma dimidiata, belong to the same suborder Heteroptera, but different from two suborders, Auchenorrhynca and Sternorrhyncha, including various gene rearrangements. Analyzing phylogenetic relationship and comparing gene order and content of the 13 hemipteran mitochondrial genomes of three suborders, Heteroptera, Auchenorrhynca, and Sternorrhyncha, supported the morphology-based current hypothesis that both Auchenorrhynca and Sternorrhyncha are a monophyletic group.
DNA-based technology are about to revolutionize the analysis of population structures as well as the determination of individual indentities. Furthermore, the analysis of polymorphic DNA regions make it possible to reach detailed conclusions on family relationships of individual. Microsatellite loci are increasingly used in population genetic and evolutionary studies. Simple sequence repeats (SSRs) or microsatellites consisted of short tandem repeats (usually 1-6 nucleotide) have shown advantages over other markers. We report here the isolation and characterization of nine highly polymerphic microsatellite loci for phylogenetic and population genetic use in silkworm. Comparative analysis of diverse silkworm strains with microsatellite locus revealed several alleles and discriminative heterozygosity values. A list of primer sequences that tag each locus is provided. The usefulness of microsatellite markers can be expected to enhance the classification in silkworm.
The genus Linaeidea Motschulsky, 1860, contains six species, and is distributed in China, Japan, USSR, Europe, with only two species known in Korea (Gressitt and Kimoto, 1963; Seeno and Wilcox, 1982; Kimoto and Takizawa, 1994; Lee and An, 2001). Morphological notes of the immature stages and life history of Japanese L. aenea (Linne, 1758) were well studied by Kimoto (1962)and Kimoto and Takizawa (1994).
Very little is known about the immature stages of this genus from Korea: only the larva of L. aenea have been briefly described and illustrated by Lee (1996).
According to Hennig (1938), this genus is separable from the genus Chrysomela, in having sternal tubercles which are disappeared in the last instar larvae. However, the L. adamsi was not applied in the diagnosis character of this genus.
The purpose of this results are to provide a key, detailed description, illustration and tubercles patterns of all known Korean species of genus Linaeidea as the basic data for the phylogenetic study the subfamily Chrysomelinae.
The 15,389-bp long complete mitochondrial genome (mitogenome) of the redspotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, but differs from the most common type, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. No typical start codon of the P. bremeri COI gene is available. Instead, a tetranucleotide, TTAG, which is found at the beginning context of all sequenced lepidopteran insects was tentatively designated as the start codon for P. bremeri COI gene. All protein-coding genes (PCGs), but COII (T) have complete termination codon TAA or TAG. One of the most unusual feature of the P. bremeri mitochondrial genome is the presence of two tRNA-like structures, such as the tRNATrp-like sequence and tRNALeu (UUR)-like sequence with proper anticodon and clover-leaf structures in the 514-bp long A+T-rich region. Furthermore, the A+T-rich region possesses three sequences that have the potential to form stem-and-loop structures, flanked by the conserved sequences, "TA(A)TA" at the 5’ end and "G(A)nT’ at the 3’ end. After more genomic and phylogenetic analyses are performed, further detailed information will be available.
In this study, we determined the complete mitochondrial genome of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae), from four overlapping fragments. The 15,592-bp long C. fulgidissima mitogenome exhibits a gene arrangement and content identical to the most common type in insects. The start codon of the C. fulgidissima COI gene is unusual, in that no typical ATN codon is available. The 875-bp A+T-rich region is the shortest among the coleopteran mitogenomes that have thus far been sequenced in their entirety. The most unusual feature of the genome is the presence of three tRNA-like sequences within the A+T-rich region: two tRNALeu(UUR)-like sequences and one tRNAAsnlike sequence. These sequence stretches evidence the proper anticodon sequence and the potential to form secondary structures, but also harbor many mismatches in the stems. Phylogenetic analysis using a concatenation of 13 amino acid sequences of protein-coding genes among the available sequenced species of coleopteran superfamilies (Buprestoidea and Elateroidea belonging to the infraorder Elateriformnia, and Chrysomeloidea and Tenebrioroidea belonging to the infraorder Cucujoiformia) by Bayesian inference, maximum-parsimony analyses, and maximum-likelihood analysis unexpectedly revealed a lack of support for monophyletic Elateriformia.
We determined the complete mitochondrial genome of the yellow-spotted long horned beetle, Psacothea hilaris (Coleoptera: Cerambycidae) that is an endangered insect species in Korea. The genome was sequenced from four overlapping fragments: two short fragments and two long fragments. The 15,857-bp long P. hilaris mitochondrial genome has the gene content typical of animal mitochondrial genome: 13 protein-coding genes, 22 tRNA genes, 2 ribosomal genes, and one non-coding A+T-rich region. The gene arrangement of the molecule is identical to the most common type found among insect mitochondrial gene arrangement that is regarded as ancestral for insects. Like several other coleopteran species the P. hilaris COI gene has typical mitochondrial start codon ATT. The 1,190-bp long A+T-rich region contains 57-bp long seven identical repeat sequences and at least seven stem-and-loop structures, composed of stems with perfect matches and loops with variable size. All P. hilaris tRNAs can be folded into the typical clover-leaf structure, with the exception of tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. After more genomic and phylogenetic analyses are performed, further detailed information will be available.
Lycorma delicatula, once mistakenly reported its occurrence in Korea, is now suddenly common in western Korea, due to its recent arrival from China and their subsequent settlement. A history of name changes in two fulgorid species, Lycorma delicatula and Limois emelianovi is reviewed. We propose to use 꽃매미 instead of its temporary name, 주홍날개꽃매미 for Lycorma delicatula, and, based on the ICZN code 32.5.1, to use Limois emelianovi instead of Limois emeljanovi for 희조꽃매미.
A recent study on Neuroptera brought us an attention to a newly found group, Coniopterygidae, dustywings. As we reported for the first time this year, this family has not been taxonomically reported in South Korea while it has been reported in North Korea before. In fact, this is known to be found in Japan and China, which means this probably have been around us for a long time. However, we found there was one species of which the name was once mentioned in a paper in 1978. It was reported by Kim, H.S. et al. in 1978 in a study of citrus red mite and its natural enemies (Kim et al., 1978). Although the spelling of the species was wrong even as a synonym, the species was found to be a natural enemy of citrus red mite, Panonychus citri in Jeju-do. We here report this taxonomically undescribed species for the first time in South Korea. The species is superficially similar to white flies but, unlike white flies, it is on our side as a natural enemy.
To understand geographic genetic variation of the species and relationships among populations of the bumble bee, Bombus ardens, that is utilized as green house pollinator we expanded our investigation by sequencing somewhat longer mitochondrial DNA (mtDNA) fragment, covering some uninvestigated regions within the species distribution, and analyzing the sequence data in terms of population genetic structure. For the purpose of study, a portion of mitochondrial COI gene, corresponding to "DNA Barcode" region (658 bp) was sequenced from 160 individuals of B. ardens collected over 15 localities in Korea. The sequence data revealed overall relatively low genetic diversity within species, with a maximum sequence divergence of 0.3%. Geographically, one haplotype (BARBA01) was found in all localities surveyed, with the frequency of 91% (145 among 160 individuals), whereas other haplotypes were found in a locality mostly as a single individual, suggesting that haplotype distribution can be summarized as coexistence of widespread, one dominant haplotype and regionally restricted, other haplotypes. Overall, very high rate of per generation female migration (Nm = 4.6 ~ infinite) and very low level of geographic substitution (FST = 0 ~ 0.099) among localities were characteristic. Although some populations were genetically subdivided from the remaining localities in the hierarchical analysis, there was regional polarity on this subdivision. Taken together with gene flow estimates, the nature of genetic divergence of the bumble bee populations is characterized as one that possessing low genetic diversity, high gene flow, and wide spread of one dominant haplotype, consistent with the previous finding. To have further detailed information of this valuable genetic resource, further longer and variable molecular portion is under investigating.
The 15,338-bp long complete mitochondrial genome (mitogenome) of the Japanese oak silkmoth, Antheraeayamamai (Lepidoptera: Saturniidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, but differs from the most common type, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. No typical start codon of the A. yamamai COI gene is available. Instead, a tetranucleotide, TTAG, which is found at the beginning context of all sequenced lepidopteran insects was tentatively designated as the start codon for A. yamamai COI gene. Three of the 13 protein-coding genes (PCGs) harbor the incomplete termination codon, T or TA. All tRNAs formed stable stem-and-loop structures, with the exception of tRNASer(AGN), the DHU arm of which formed a simple loop as has been observed in many other metazoan mt tRNASer(AGN). The 334-bp long A+T-rich region is noteworthy in that it harbors tRNA-likestructures, as has also been seen in the A+T-rich regions of other insect mitogenomes. Phylogenetic analyses of the available species of Bombycoidea, Pyraloidea, and Tortricidea bolstered the current morphology-based hypothesis that Bombycoidea and Pyraloidea are monophyletic (Obtectomera). As has been previously suggested, Bombycidae (Bombyxmori and B.mandarina) and Saturniidae (A.yamamai and Caligula boisduvalii) formed a reciprocal monophyletic group.