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Comparison of the morphological clustering and molecular phylogeny of the tribe Aphidini (Hemiptera: Aphididae)
Multivariate morphomatric analyses were conducted to cluster the morphologically similar group using species units within the tribe Aphidini. Some species of the genus Aphis are morphologically very similar each other and, mentioned by aphid taxonomists as well, possibly grouped by some characteristics. To cluster the morphologically related groups and find some significant characteristics to define morphological groups for 59 Aphidini species, we perform two statistical analyses of 30 morphomatric characters, Principle Component Analysis (PCA; SAS Procedure PRINCOMP) and Canonical Discriminant Analysis (CDA; SAS Procedure CANDISC) using SAS/STAT version 9.1.3 (SAS Institute, Inc., Cary, North Carolina). The results of the morphological clustering analyses were compared with the molecular phylogeny of Aphidini obtained from the previous study using three molecular gene fragments: partial mitochondrial tRNA-leucine + cytochrome oxidase II (tRNA/COII), partial mitochondrial 12S + tRNA-valine + 16S (12S/16S), and nuclear elongation factor-1 alpha (EF1α) (total 3,289bp) for 37 representative species among them. The congruence or difference between morphological and molecular analyses is also discussed in Aphidini group
