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Isolation and Molecular Cloning of Novel Venom Peptides from Orancistrocerus drewseni (Hymenoptera: Eumenidae)
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/290662
Three venom peptides, OdVP1, OdVP2, and OdVP3 were isolated from the venom of the solitary wasp Orancistrocerus drewseni (Hymenoptera: Eumenidae). The venom peptide amino acid sequences were determined by Q-TOF/MS/MS. The OdVP1, 2, and 3 with amidated C-terminals showed similar peptide sequences to the mastoparan from Vespula lewisii or the protonectin from Protonectarina sylveirae, suggesting that they adopt an amphipathic α-helix secondary structure. The amidation of C-terminal Leu of the venom peptides have been known to be required for their biological activities. The full-length open reading frame (ORF) sequences of the OdVP1, 2, and 3 were analyzed by 5’- and 3’-rapid amplification of cDNA ends (RACE). The overall gene structure of OdVPs showed a high homology to that of mastoparan B from Vespa basalis by containing signal sequence, prosequence, mature peptide and C-terminal glycine, but the mature peptide sequences were distinct from each other. The toxicological property and antimicrobial activity of OdVPs were characterized using synthetic peptides. This study on the venom peptides from O. drewseni should promote further studies on bioactive ingredients in the venom of solitary hunting wasps.
