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Cloning and characterization of immune-related genes from the swallowtail butterfly, Papilio xuthus, using annealing control primer-based GeneFishing PCR system

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  • URLhttps://db.koreascholar.com/Article/Detail/291424
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

We have previously shown that the larvae of swallowtail butterfly, Papilio xuthus, exhibit substantial antibacterial activity in the hemolymph, upon challenging with bacterial lipopolysaccharide (LPS). Here we report the isolation and molecular characterization of several immune inducible genes that are specifically expressed by employing annealing control primer (ACP)-based GeneFishing polymerase chain reaction (PCR) from P. xuthus the larva. Using 120 arbitrary ACPs, we identified 24 differentially expressed genes (DEGs) that are up-regulated in response to injected LPS. Sequence analysis showed that 18 DEGs revelaed a high sequence similarity to the previously characterized genes of other insects, although 6 DEGs showed no significant similarity to any known genes. Among these inducible transcripts we found 8 putative immune-related genes including cecropin and attacin. Finally, we analysed the expression profiles of potential immune-related genes by RT-PCR and found all of them were considerably increased in the mRNA levels by LPS injection.

저자
  • Seong-Ryul Kim(Department of Agricultural Biology, NIAST, RDA)
  • Ha-Yan Park(Department of Agricultural Biology, NIAST, RDA)
  • Mee-Yeon Hong(Department of Agricultural Biology, NIAST, RDA, Department of Agricultural Biology, Chonnam National University)
  • Kwan-Ho Park(Department of Agricultural Biology, NIAST, RDA)
  • Hwa-Jin Suh(Department of Agricultural Biology, NIAST, RDA)
  • Iksoo Kim(Department of Agricultural Biology, Chonnam National University)
  • Jae-Sam Hwang(Department of Agricultural Biology, NIAST, RDA)