Pro-inflammatory cytokines are important mediators of cutaneous cellular activities during many oral mucosal diseases. IHOK culture model transfected by E6/E7 genes provide further evidence for the role of HPV in tumorogenesis. It is interesting to investigate cytokine expression of immortalized human oral keratinocyte(IHOK). The purpose of this study were to analysis cytokine mRNA expression levels of NHOK and IHOK by RT-PCR. IHOK showed about 5 fold increases of IL-6 compared with NHOK, while TNF-α was the lowest. It suggested that immortalization of NHOK with E6/E7 could result in elevated expression of IL-6, and IHOK be in the intermediate stage of oral carcinogenesis.

I. INTRODUCTION
 II. MATERIALS and METHODS
  1. Cell culture condition
  2. Total RNA extraction from NHOK and IHOKfor RT-PCR
  3. RT-PCR reaction for cDNA
 III. RESULTS
 IV. DISCUSSION
 REFERENCES

• 이종헌(단국대학교 치과대학 구강병리학교실) | Chong Heon Lee correspondence
• 김산(첨단과학대학 미생물학전공) | San Kim