ISPARC (Secreted protein acidic and rich in cysteine) is detected in the bone stroma during wound-healing process. To understand the roles of SPARC in bony wound-healing process, SPARC cDNA were synthesized from rat calvarial osteoblast culture, and SPARC protein was synthesized from the cDNA. To observe the effects of SPARC protein on the differentiation of osteoblasts, bony defect were made on rat tibia, and the distributions of bone matrix related proteins and SPARC were investigated using immunohistochemistry. In the rat osteoblastic culture using untreated plastic surface, Collagen-SPARC treated surface presented higher protein synthesis than untreated surface or only collagen treated surface. SPARC synthesis in the bony defect of rat tibia was augmented by introducing SPARC to the bony defect. SPARC synthesis were increased from the center of the defect compared to the control. SPARC synthesis in cells of the center of the defect was increased and maintained for 14 days. We could conclude that SPARC introduction may affect the early bone matrix formation, including SPARC, and mineralization in bony wound healing process.