This research was designed to investigate changes of growth factors and bone matrix proteins during the bone healing processes using immunohistochemistry and in situ hybridization. Especially this study was focused on the changes of bone matrix and growth factors around the titanium implant. Threaded implants were introduced into the long bone of tibia. Time dependent changes of several bone associated protein and and its mRNAs were observed. Proteins investigated in this study are collagen, osteonectin(ON), osteopontin(OPN), osteocalcin(OC). Expression of the proteins were measured using immunohistochemistry. VEGF and ON were measured using in situ hybridization, and northen blot technique. Bone regeneration were observed as early as the third day of experiment. Matrix proteins and growth factors observed around implant were identical to the proteins observed in the control group. The expression of the ON, OC and VEGF were observed mainly in the osteoblast-like cell on the surface of new bone around the implant and the cells lining the margin of bone defect apart from the implant. The observation may not result from direct osteoconducting activities of titanium but by passive adsorption of extracellular factors which has bone inducing capacities. These passive adsorption results in the immobilization of the growth factors and consequent prolongation of the activities.

I. 서론
 II. 연구재료 및 방법
  1) 연구방법
  2) 식립할 임플란트의 제작
  3) 수술 및 표본제작
  4) 면역조직화학염색
  5) 조직계측학적 검사
 III. 연구결과
  1) 조직형태계측학적 분석
  2) 광학현미경적 소견
  3) 면역조직화학적 관찰
 VI. 총괄 및 고찰
 V. 참고문헌

• 문형주(서울대학교 치의학대학원 구강병리학교실) | Hyung Ju Moon
• 홍성두(서울대학교 치의학대학원 구강병리학교실) | Seong Doo Hong
• 홍삼표(서울대학교 치의학대학원 구강병리학교실) | Sam Pyo Hong
• 임창윤(서울대학교 치의학대학원 구강병리학교실) | Chang Yun Lim
• 이재일(서울대학교 치의학대학원 구강병리학교실) | Jae Il Lee correspondence