논문 상세보기
pp.121-128
Amino acid transporters are essential for the growth and proliferation in all living cells. Among the amino acid transporters, the system L amino acid transporters are the major nutrient transport system responsible for the Na+-independent transport of neutral amino acids including several essential amino acids. The L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed in cancer cells to support their continuous growth and proliferation. 2-Aminobicyclo-(2,2,1)-heptane-2-carboxylic acid(BCH) is a model compound for study of amino acid transporter as a system L selective inhibitor. We have examined the effect and mechanism of BCH on cell growth suppression in FaDu human head and neck squamous cell carcinoma. The BCH inhibited the L-leucine transport in a concentration-dependent manner with a IC 50 value of 43.8±4.3μM. The majority of L-leucine uptake is, therefore, mediated by LAT1 in FaDu cells. The growth of FaDu cells was inhibited by BCH in the time- and concentration- dependent manners. The formation of DNA ladder was not observed with BCH treatment in the cells. Furthermore, the proteolytic processing of caspase-3 and caspase-7 in the cells was not detected by BCH treatment. These results suggest that the BCH inhibits the growth of FaDu human head and neck squamous cell carcinoma through the intracellular depletion of neutral amino acids for cell growth without apoptotic processing
II. 재료 및 방법
1. 실험재료 및 세포주
2. Uptake 실험
3. MTT 분석
4. DNA fragmentation 분석
5. Immunoblotting
6. 실험자료의 통계학적 검정
III. 결과
1. L-leucine 수송에 영향을 미치는 BCH의 효과
2. 세포성장에 영향을 미치는 BCH의 효과
3. DNA fragmentation 분석
4. BCH에 의한 caspase의 활성
IV. 고찰
V. 결론
VI. 참고문헌
