We have previously shown that 5’-nitro-indirubinoxime (5’-NIO) has potent anti-tumor effect in various human cancer cells. But the potential anti-invasive effect of 5’-NIO in salivary gland cancer has not been studied yet. The goal of this study is to evaluate the effect of 5'-NIO on salivary gland adenocarcinoma SGT cell adhesion and migration to Type I collagen treatment. Western blot, adhesion and migration assay were performed to evaluate the impacts of 5’-NIO on the expression of MMP-2/-9 and its upstream signaling molecules after treatment of type І collagen. SGT cell adhesion to type I collagen is significantly suppressed by 5’-NIO. 5’-NIO decreased expression of β1 integrin, phosphorylation of FAK, MMP-2/ -9 compared with type I collagen treatment. In addition, 5’-NIO inhibited the migration of SGT cells treated with type I collagen. These results suggest that 5’-NIO could effectively inhibit the invasion and migration of human SGT cells by downregulating the expression of β1 integrin and MMP-2/-9 and phosphorylation of FAK, Akt, and Erk. Adhesion and migration to type I collagen of SGT cells can be influenced through 5’-NIO..

I. 서론
 II. 연구재료 및 방법
  1. 세포 배양 및 MTT 분석
  2. 세포부착 실험(Cell adhesion assay)
  3. 세포이동 실험(Cell migration assay)
  4. Western blot analysis
  5. Statistical analysis
 III. 결과
  1. SGT 세포의 type I collagen 부착에 대한5’-NIO의 영향
  2. Type I collagen 처리 후 단백질 발현 변화
  3. Type I collagen 처리 후 SGT 세포 이동에서5’- NIO의 효과
 IV. 고찰

• 윤정훈(조선대학교 치과대학 병리학교실) | Jung Hoon Yoon correspondence
• 안상건(조선대학교 치과대학 병리학교실) | Sang Gun Ahn correspondence