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pp.20-26
In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate thepossibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves wereprepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in etha-nol extract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction(661.38㎎/g), butanol fraction (396.68㎎/g) and 80% ethanolic extract (239.54㎎/g) has higher total polyphenol contentsthan other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. Theethyl acetate fraction showed the highest levels of DPPH radical scavenging activity (IC50, 4.77㎍/㎖). Moreover, the ethylacetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxic-ity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate frac-tion, have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate fornatural and functional materials.
