This study was to investigate anti-skin wrinkle effect of Agastache rugosa Kentz extracts by extraction processes.In the comparison of the effect of the solvent extraction, the extracts by 70% ethanol (EE) showed better biological activitiesthat those by hot water. Therefore, further fermented Agastache rugosa was applied to 70% ethanol extraction process(FEE). FEE showed higher DPPH scavenging activity of 62.98% than EE’s 62.71% at 1.0㎎/㎖, but there was no significant.Elastase inhibition was measured 23.0% from FEE at 1.0㎎/㎖. Cytotoxicity showed the highest 16.26% from FEE, thisvalue is safe in the cell experiment. Collagen production showed 113.1ng/㎖ from FEE, on the other hand EE was measured77.4ng/㎖ in adding 1.0㎎/㎖. MMP-1 production was observed 1398pg/㎖ from FEE and EE was measured1632pg/㎖. These results were found the highest antioxidant and anti-wrinkle effect. As a result, it was also confirmed thatanti-skin wrinkle activities of the Agastache rugosa Kentz extract was correlated with anti-oxidant activities.

 서 언  재료 및 방법   1. 시료 제조   2. 세포 및 시약   3. DPPH free radical scavenging activity   4. Elastase 저해율 측정   5. MTT용액을 이용한 세포 독성평가   6. Human dermal fibroblast (HDF)를 이용한 collagen생성 실험   7. MMP-1 (Matrix metalloproteinase-1) 생성 실험   8. 통계처리  결과 및 고찰   1. DPPH free radical scavenging activity   2. Elastase 저해율 측정   3. MTT용액을 이용한 세포 독성평가   4. Human dermal fibroblast (HDF)를 이용한 collagen생성 실험   5. MMP-1 (Matrix metalloproteinase-1) 생성 실험  감사의 글  REFERENCES

• 김남영(강원대학교 생물의소재공학과) | Kim, Nam Young
• 박대수( 서원대학교 식품공학과) | 박대수
• 이현용( 서원대학교 식품공학과) | 이현용