논문 상세보기

Fine mapping of the potyvirus reistance gene Pvr7 in C. annuum

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/297707
서비스가 종료되어 열람이 제한될 수 있습니다.
한국육종학회 (The Korean Breeding Society)
초록

Pepper mottle virus (PepMoV) is frequently occurring virus in pepper field. PepMoV infected plants show symptoms including mosaic leaf, distortion of foliage and fruit deformation. The dominant gene Pvr7 from Capsicum annuum ‘9093’ confers resistance to PepMoV. Previous research reveals that Pvr7 is located in 10 chromosome and linked to the dominant potyvirus resistance gene Pvr4 and Tomato spotted wilt virus (TSWV) resistance gene Tsw. To identify the Pvr7 gene, we constructed an intraspecific F2 mapping population from a cross between C. annuum ‘9093’ (PepMov resistant) and C. annuum ‘Jejujaerae’ (PepMoV susceptible). Resistance of F2 plants were screened with green flouorescent protein (GFP) tagged PepMoV. Genomic DNA was extracted from F2 individuals and markers were developed using C.annuum ‘CM334’ whole genome sequence (WGS). Several single nucleotide polymorphism (SNP) markers that were co-segregated with Pvr7 were developed. We are expecting that this Pvr7 SNP marker can be used to breeding PepMoV resistant cultivars and fine mapping of Pvr7.

저자
  • Jeong-Tak An(Dept. of Plant Science)
  • Won-Hee Kang(Dept. of Plant Science)
  • Saet-Byul Kim(Dept. of Plant Science)
  • Jin-Kyung Kwon(Dept. of Plant Science)
  • Doil Choi(Dept. of Plant Science, Plant Genomics and Breeding Institute, College of Agricultural and Life Sciences, Seoul National University)
  • Byoung-Cheorl Kang(Dept. of Plant Science, Plant Genomics and Breeding Institute, College of Agricultural and Life Sciences, Seoul National University) Corresponding Author