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Effects of air temperature and light intensity on the acquisition of thermal image of rice (Oryza sativa L.)
It is assumed that air temperature and light intensity may influence thermal image of plants but little effort has been made to these environmental factors. We conducted this study to investigate the effects of these environmental factors on the thermal image of rice and thus to optimize the condition for thermal image acquisition for high-throughput screening of salt-tolerant rice. Rice (Oryza sativa cv. Chucheong-byeo) seedlings at the four-leaf stage were treated with 0, 50, and 100 mM of NaCl for salt stress. Thermal images (T420, FLIR, Sweden) were taken at 1 and 2 days after salt treatment under 4 different air temperatures and 3 light intensities. Thermal images were analyzed using FLIR Tools 3.1 (FLIR systems Inc., USA) and MATLAB 8.1 (The MathWorks Inc., USA). Rice leaf temperature increased significantly with increasing air temperature and light intensity, resulting in greater discrimination between salt-stressed and unstressed rice plants. Our results thus conclude that environmental conditions such as air temperature and light intensity affect rice thermal image and their optimization is essential for better image acquisition and high-throughput screening system based on thermal image analysis
