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Variation and haplotypes of NaCl salinity on rice at germination stage based on Genome-wide association study

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  • URLhttps://db.koreascholar.com/Article/Detail/297943
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한국육종학회 (The Korean Breeding Society)
초록

Salt toxicity is the major factor limiting crop productivity in saline soils. Rice is an important staple food crop of nearly half of the world population and is well known to be a salt sensitive crop. The completion and enhanced annotations of rice genome sequence has provided the opportunity to study functional genomics of rice. With the rapid development of the biotechnology techniques, we can use more accurate and reliable methods to study the mechanism and function in different stress conditions. In present study, 295 rice accessions of diverse origin were re-sequenced and used for genome-wide association study (GWAS) with several germination-related traits, including germination percentage (GP), germination energy (GE), germination rate (GR), germination index (GI), salt tolerance index (STI) in salt tolerant germination stage. Phenotyping of the rice accessions were carried out at 200mM NaCl to screen salt tolerance levels. GWAS was applied to detect the associated genes related to salt tolerance in rice germination stage. Variations and haplotypes of the associated genes were detected and correlation between the phenotypes and genotypes were validated using qRT-PCR.

저자
  • Jie Yu(Department of Plant Resources, College of Industrial Sciences, Kongju National University)
  • Tae-Sung Kim(Department of Plant Resources, College of Industrial Sciences, Kongju National University)
  • Yong-Jin Park(Department of Plant Resources, College of Industrial Sciences, Kongju National University, Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) Corresponding Author