Based on the results of microarray analysis we selected ten candidate genes that express in pollen at the early pollen developmental stage. By PCR amplification, the promoter region of these genes were amplified from rice genomic DNA (Nipponbare) and cloned into the destination pKGWFS7 vector via an entry vector, pDONR201. The characteristic of promoters were evaluated in Arabidopsis thaliana (Col-0) through GUS expression analysis. Fifty T2 plants respectively from each promoter were tested. Whole inflorescence of individual plant was stained with 1mM X-Gluc solution to observe tissue-specific GUS expression patterns. The results showed that all 10 promoters activated in pollen tissues. Among them six promoters expressed at the early developmental stage (unicellular) of pollen and the others expressed at both early (unicellular) and late pollen developmental stage (mature pollen). The results indicated that these promoters would be potential applicable for the studies of pollen function. Currently, we are performing these promoters analysis in rice transgenic plants as well as molecular characterization.

• Tien Dung Nguyen(School of Applied Biosciences, Kyungpook National University)
• Moe Moe Oo(School of Applied Biosciences, Kyungpook National University)
• Sung Aeong Oh(School of Applied Biosciences, Kyungpook National University)
• Thi Hoai Thuong Nguyen(School of Applied Biosciences, Kyungpook National University)
• Hyo-Jin Park(School of Applied Biosciences, Kyungpook National University)
• Jong Tae Song(School of Applied Biosciences, Kyungpook National University)
• Moon-Soo Soh(Department of Molecular Biology, Sejong Universityy)
• Soon Ki Park(School of Applied Biosciences, Kyungpook National University) Corresponding Author