2-acetyl-1-pyrroline (2AP) was widely known as the principal aroma compound, it development in rice has been reported due to the loss of function of betaine aldehyde dehydrogenase gene (badh2) on chromosome 8. In previous study, a lot of haplotypes have been found of this gene, while only limited haplotypes have been proved as functional mutations. A total of 137 core set accessions, and additional 45 germplasms have been employed in this study. Finally, two new mutations have been found (3bp deletion in exon12 and C/A SNP in exon 10), and 23 haplotypes have been detected, most of them had strong relations with aroma formation. According to the sequence results, five functional markers have been developed, the markers showed a highly efficient in discriminating the special aromatic rice varieties, and displayed perfect co-segregation with the trait of fragrance in F2 population. Those new markers developed in the present study would be useful in molecular breeding of fragrant rice varieties. Based on the haplotypes, the further research is in progress.

• Qiang He(Department of Plant Resources, College of Industrial Sciences, Kongju National University)
• Yoo-Hyun Cho(Department of Plant Resources, College of Industrial Sciences, Kongju National University, Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University)
• Tae-Sung Kim(Department of Plant Resources, College of Industrial Sciences, Kongju National University)
• Young-Sang Lee(Department of Medical Biotechnology, Soonchunhyang University)
• Yong-Jin Park(Department of Plant Resources, College of Industrial Sciences, Kongju National University, Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) Corresponding Author