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Production of soybean transgenic plants with Bt crystal protein genes

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/297993
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한국육종학회 (The Korean Breeding Society)
초록

Bacillus thuringiensis (Bt) crystal protein genes encode insecticidal δ-endotoxins that are widely used for the development of insect-resistant crops. Common soybean is a crop of economic and nutritious importance in many parts of the world. Korea soybean variety Kwangan was transformed with Bacillus thuringiensis (Bt) crystal protein genes. These genes were transformed into Kwangan using highly efficient soybean transformation system. Transgenic plants with Bt crystal protein genes were confirmed for gene introduction and their expression using PCR, real-time PCR and RT-PCR. Currently, the confirmation of stable gene introduction with Bt genes is also performing by southern blot analysis and physiology test and agronomic characters are investigating.

저자
  • Ha Neui Hong(Dept. of Genetic Engineering, Dong-A University)
  • Jung Hun Pak(Dept. of Genetic Engineering, Dong-A University)
  • Mi-Jin Kim(Dept. of Genetic Engineering, Dong-A University)
  • Hye Jeong Kim(Dept. of Genetic Engineering, Dong-A University)
  • Quyen Nguyen Thi(Dept. of Genetic Engineering, Dong-A University)
  • Yeon Ho Je(Dept. of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Young Soo Chung(Dept. of Genetic Engineering, Dong-A University) Corresponding Author