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Development of EST-SSR marker for MAS breeding with populations to resistant at white-backed planthopper (Sogatella furcifera Horvath)

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  • URLhttps://db.koreascholar.com/Article/Detail/298047
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한국육종학회 (The Korean Breeding Society)
초록

This study was carried out to develop expressed sequence tag-Simple sequence repeat (EST-SSR) markers of brown plant hopper resistance gene originated from a rice cultivar ‘Cheongcheong’ and sensitive rice cultivar ‘Nakdong’. Total RNA extracted from the leaves of ‘Cheongcheong’ and ‘Nakdong’ were used to synthesize a cDNA library. As a result of analyzing the cDNA library, EST-SSR sites were found and the EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice cultivar more simply, quickly and precisely. Furthermore, using this marker’s advantage of deriving from cDNA, it is possible to identity the white-backed planthopper resistance gene.

저자
  • Kyung-A Kim(Department of Biology, College of Natural Sciences, Kyungpook National University)
  • Jae-Keun Sohn(School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University)
  • Kyung-Min Kim(School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University) Corresponding Author