Korean soybean variety Kwangan was transformed with coat protein (CP), helper component-proteinase (HC-Pro), and ABRE binding factor 3 (ABF3) genes using highly efficient soybean transformation system. Among these genes, CP and HC-Pro were transformed using RNAi technology. Transgenic plants with CP were confirmed for gene introduction and their expression using PCR, real-time PCR, RT-PCR, Southern blot, and Northern blot. To investigate the response of viral infection with CP, T1 plants were inoculated with SMV-infected leaves and confirmed the existence of mosaic symptom in both leaves and seeds. Two transgenic lines with CP were highly resistant to SMV with clear leaves and seeds while SMV-susceptible lines showed mosaic symptom with seed mottling. The transcript levels of T1 plants with CP were also determined by northern blot, suggesting that SMV-resistant T1 plants did not show viral RNA expression whereas SMV-susceptible T1 plants showed viral RNA expression. Currently, the response of viral infection with HC-Pro is investigating to produce SMV-resistant soybean transgenic plants, and the physiological experiment with ABF3 is also carrying out to produce drought-tolerant soybean transgenic plants.

• Hye Jeong Kim(Dept. of Genetic Engineering, Dong-A University)
• Jung Hun Pak(Dept. of Genetic Engineering, Dong-A University)
• Mi-Jin Kim(Dept. of Genetic Engineering, Dong-A University)
• Ha Neui Hong(Dept. of Genetic Engineering, Dong-A University)
• Quyen Nguyen Thi(Dept. of Genetic Engineering, Dong-A University)
• Yeong Hoon Lee(National Institute of Crop Science, RDA)
• Ki Hyun Ryu(Division of Environmental and Life Sciences, Seoul Women’s University)
• Kook-Hyung Kim(Dept. of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University)
• Soo Young Kim(Kumho Life and Environmental Science Laboratory)
• Young Soo Chung(Dept. of Genetic Engineering, Dong-A University) Corresponding Author