Sound and communication through it have significantly contributed to study the ecology, evolution, behavior in animal. Plants may also use sound, but until now, we have been unable to effectively research what the ecological, evolutionary and molecular implications might be in plant. So, we wonder what genes are regulated under sound wave conditions. In particular, our research was centered to increase functional materials including vitamins and anthocyanin in plants. First, we investigated up- and down-regulated genes under sound wave treatments (250, 500, 800, 1000 and 1500Hz) by RNA-seq in Arabidopsis thaliana. In these results, we selected genes of over 8-fold increase and below 8-fold decrease and especially, focus on vitamin and anthocyanin-related genes in RNA-seq level. Second, we confirmed that these up- and down-regulated genes under sound wave treatments by qRT-PCR. Finally, we selected 13 interesting genes. To confirm these results, now, we are performing promoter assay by using promoter-GUS in plant and by using promoter-luciferase in protoplast. After then, we will find to interacting partners of these genes in sound wave signal. Our final goal is understand signaling network under sound wave treatment condition in plant. We hope that if we do find results that suggest that sound wave have a beneficial effect on crop yield and quality, acoustic biology can then have some viable application in agriculture. This could bring new discoveries into development of farming methods.

• Joo-Yeol Kim(National Institute of Agricultural Biotechnology, Rural Development Administration)
• Gyu-Myuong Lee(National Institute of Agricultural Biotechnology, Rural Development Administration)
• Soo-In Lee(National Institute of Agricultural Biotechnology, Rural Development Administration)
• Jin-A Kim(National Institute of Agricultural Biotechnology, Rural Development Administration)
• Mi-Jeong Jeong(National Institute of Agricultural Biotechnology, Rural Development Administration) Corresponding Author