Transposon elements are widely distributed in the plant genomes. Maize genome consists of various transposable elements that constitute as much as 80% of the genome. Transposon display (TD) is a modified from AFLP, and can be used to generate and display hundreds of genomic fragments affixed to transposons, consequently tagging transposons. We developed maize specific transposon insertion- sequence characterized amplified regions (Ti-SCAR) using Isaac and CACTA transposons. Currently, we have developed 58 dominant Ti-SCAR markers. Validation of the Ti-SCARs is being carried out using a various maize germplasm. Since AFLP is tedious and unsuitable for large scale application in MAS, the Ti-SCAR markers displayed simple binary presence or absence pattern. Thus, the Ti-SCARs can provide a fast, cheap and reliable PCR based assay. A pipeline in developing the Ti-SCAR will be presented in the poster.

• Neha Roy(Department of Molecular Bioscience)
• Ji-Young Choi(Department of Molecular Bioscience)
• Sung-Il Lee(Department of Molecular Bioscience)
• Min-Ji Lim(Department of Molecular Bioscience)
• Kyong-Cheul Park(Department of Agricultural Life Science, Kangwon National University)
• Nam-Soo Kim(Department of Molecular Bioscience)