This study investigated saponin contents according to collection area, seed size and seed color in 293 Korean soybean landraces. Results showed that total group A was 149.8 to 1279.0㎍/g with an average of 484.9㎍/g while total group B saponin content was 2160.1 to 7868㎍/g with an average of 3670㎍/g. Total groups A and B saponin content were ranging from 2502.7 to 8763.9㎍/g with an average of 4154.8㎍/g. The landraces with high group B saponin content were IT226841, IT226761, IT226844 and IT226826. The landraces with low group A saponin content were IT228580, IT228319, IT228308 and IT226841. According to collection areas, the highest amount of saponin content was observed in the landraces collected from North Korea. High total of saponin content was showed by seeds from Gyeonggi-do, followed by Chungcheongbuk-do, Gangwon-do, Gyongsangbuk-do, Chungcheongnam-do Jeollabuk-do and Jeollanam-do. According to seed size, small seeds have the higest saponin contentfollowed by the medium and large size of seeds. For seed color, there was no significant difference in saponin content. The total of saponin content was postively correlated with group A saponin, group B saponin and total of saponin cntent. Among each components, group A saponin showed postive correlations with total of saponin and group B saponin content.