UVB radiation which causes dermatosis, cancroid, and necrosis in living organisms is mostly absorbed by ozone layer, resulting in transmission of only small UVB proportion to earth surface. Recently, however, rapid increases of pollutants like CFCs have accelerated depletion of stratospheric ozone layer. Increased UVB irradiation alters affects biomolecule interinity such as DNA, RNA and protein. To understand DNA mutation spectra in response to UVB, in the present study, we used two soybean cultivars, Buseok and Cheongja 3, which were screened as the most UVB tolerant and sensitive genotypes among 140 soybean germplasms, respectively. Whole genomes of Buseok and Cheongja were sequenced at low-coverage depth by illumina Hiseq2000, and we also sequenced 6 hr UVB irradiated genomes of two cultivars. Raw sequence reads were aligned to the soybean reference sequences (cv. Williams 82) by BWA aligner software. To identify DNA mutations induced by UV-B irradiation, multiple comparisons between non-irradiated and irradiated genomes in these two soybean genotypes were conducted using samtools and GenomeAnalyzerTK packages and homebrew python codes. A total, 13,992 and 17,078 single nucleotide polymorphisms (SNPs) were indentified between non-irradiated and irradiated genomes of Buseok and Cheongja 3, respectively. In addition, Buseok and Cheongja 3 have 423 and 465 insertions/deletions induced by UVB, respectively. Approximately 58% of the identified SNPs were C to T or CC to TT transversions, consistent with the previous studies. Chromosomal distributions of the SNPs likely showed differences in UV-B mutation positions depending on the soybean genotype

• Sangrea Shim(Department of Plant Science and Research Institute of Agriculture and life Sciences, Seoul National University)
• Moon Young Kim(Department of Plant Science and Research Institute of Agriculture and life Sciences, Seoul National University, Plant Genomic and Breeding Research Institute, Seoul National University)
• Suk-Ha Lee(Department of Plant Science and Research Institute of Agriculture and life Sciences, Seoul National University, Plant Genomic and Breeding Research Institute, Seoul National University)