Korea is a origin of three basic species, P. ussuriensis, P. pyrifolia and P. fauriei. Genetic relationship among Korean pear cultivars compared with their parents were also identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrid between two species. SSRs or Microsatellites are co-dominant and typically neutral inheritance showing high degree of polymorphism, large number of alleles per locus, abundance in genomes, and suitability for automation. SSR markers were developed in apple and pear where they were used for construction of genetic linkage maps, evaluation of the genetic diversity, cultivar identification, genotype identification, and in the determination of genetic relatedness. Many apple (Malus × domestica Borkh.) SSRs would be useful for genetic mapping in European and Asian pears in previous experiments and cross-species amplification was observed between apple and pear. The objectives of this study were to develop polymorphic SSR markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis in each among Korean pears, from ‘Golden Delicious’ genomic sequences generated by next generation sequencing technology and to evaluate the utility of the SSR markers based on ‘Golden Delicious’ sequences. Of 51 SSR markers, 18 were polymorphic in ‘Whangkeumbae’ and ‘Minibae’. The cross-species transportability of primers designed in ‘Golden Delicious’ sequences makes SSR markers more useful, given the current high level of investment in mapping the genomes of related Rosaceae.

• Jungyeon Won(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University)
• Youngjae Oh(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University)
• Seolah Kim(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University)
• Hyunsuk Shin(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University)
• Sewon Oh(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University)
• Cheol Choi(Department of Horticultural Science, Kyungpook National Universityn)
• Daeil Kim(Department of Horticulture, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Chungbuk National University) Corresponding Author