Chronic gamma irradiation can be used an alternative mutation breeding methods for induction of many useful mutants. Seedlings of purple-colored wheat plants were irradiated with wide range doses of chronic gamma-rays (20, 25, 30, 40, 50, 70, 100, 125, 150, 200, 250, 300 Gy) during 6 weeks at gamma-phytotron in the Korea Atomic Energy Research Institute, respectively. To identify the biological responses purple-colored wheat, we examined the plant height, chlorophyll, carotenoid and total anthocyanin contents in leaf. Plant growth, chlorophyll and carotenoid contents in leaf were decreased when the dose rate increased. Anthocyanin contents were increased with the increase of the radiation dose until 50 Gy treatment. To confirm the real contents of anthocyanin, we also investigated cyanidin-3-glucoside in purple-colored wheat leaf by using UPLC analysis. These results indicate that anthocyanin accumuation was observed under chronic gamma irradiation.

• Young Ha Yoon(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Min Jeong Hong(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Jin-Baek Kim(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Si-Yong Kang(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Sang Hoon Kim(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Joon-Woo Ahn(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
• Yong Weon Seo(Division of Biotechnology & Genetic Engineering, Korea University)
• Dong Sub Kim(Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) Corresponding Author