Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and 100 μg/ml of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was 50 μg/ml of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.

서 언
 재료 및 방법
  1. 실험재료
  2. 세포배양
  3. 세포 생존율
  4. TRAP 염색
  5. TRAP 활성도 분석
  6. 역전사 중합효소 연쇄반응 (RT-PCR) 분석
  7. 석회화 결절 형성 측정
  8. 통계분석
 결과 및 고찰
  1. 우슬과 인삼의 파골세포 생존율
  2. 우슬과 인삼의 TRAP 양성 파골세포 분화 억제
  3. 우슬과 인삼의 파골세포 분화 유도인자 억제
  4. 우슬과 인삼의 조골세포 분화 효과
 감사의 글
 REFERENCES

• 김진성(농촌진흥청 국립원예특작과학원 인삼특작부) | Jin Seong Kim
• 이상원(농촌진흥청 국립원예특작과학원 인삼특작부) | Sang Won Lee
• 김영옥(농촌진흥청 국립원예특작과학원 인삼특작부) | Young Ock Kim Corresponding author
• 방만석(단국대학교, 종합임상시험원, 바이오안전성 & 유효성 센터) | Man Seok Bang
• 오충훈(단국대학교, 종합임상시험원, 바이오안전성 & 유효성 센터) | Chung Hun Oh Corresponding author
• 김철태(건양대학교, 의과학대학 응급구조학과) | Chul Tae Kim