Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

Results and Discussion
  SDS-PAGE analysis
  Two-dimensional gel electrophoresis (2-DE)
 Results and Discussion
  Protein extraction and separation
  Two-dimensional electrophoresis (2-DE) as a toolfor the efficient isolation of seed storage proteinsfrom common buckwheat
 REFERENCES

• Seong-Woo Cho(Dept. of Crop Science, Chungbuk National University)
• Soo-Jeong Kwon(Dept. of Crop Science, Chungbuk National University)
• Swapan Kumar Roy(Dept. of Crop Science, Chungbuk National University)
• Sun-Hee Woo(Dept. of Crop Science, Chungbuk National University) Corresponding author