ORE7 gene incorporated into 3 different promoters including pCKLSL-35S, pCKLSL-TP and pCSENIF was transformed to Korean soybean variety Kwangan using highly efficient soybean transformation system. The gene is known to exhibit a delayed leaf senescence phenotype in Arabidopsis. Fourteen, Fifteen and nine transgenic plants were produced from pCKLSL-35S::ORE7, pCKLSL-TP::ORE7 and pCSENIF::ORE7, respectively. Moreover, transgenic plants were confirmed for gene introduction and their expression using PCR, qRT-PCR and RT-PCR. To identify the transgene insertion events, the analysis of flanking sequence was determined. As a results, T-DNA was integrated intergenically in transgenic line 1 of pCKLSL-35S::ORE7 and line 1 of pCSENIF::ORE7. Currently, flanking sequence analysis with pCKLSL-35S::ORE7, pCKLSL-TP::ORE7 and pCSENIF::ORE7 is carrying out to investigate the stable T-DNA insertions.

• Yoon Jeong Lee(Dept. of Genetic Engineering, Dong-A University)
• Hye Jeong Kim(Dept. of Genetic Engineering, Dong-A University)
• Su Yeong Yun(Dept. of Genetic Engineering, Dong-A University)
• Hyun Suk Cho(Dept. of Genetic Engineering, Dong-A University)
• Hyun Hee Im(Dept. of Genetic Engineering, Dong-A University)
• Dong Hee Lee(Venture Bldg 306 Pohang Techno Park Pohang)
• Young Soo Chung(Dept. of Genetic Engineering, Dong-A University) Corresponding Author