논문 상세보기

Production of soybean transgenic events harboring AtRabG3b and CaMsrB2 genes

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/298481
서비스가 종료되어 열람이 제한될 수 있습니다.
한국육종학회 (The Korean Breeding Society)
초록

AtRabG3b and CaMsrB2 genes incorporated into pPZP vetor were transformed to Korean soybean cultivar Kwangan using highly efficient transformation system. AtRabG3b gene plays a positive role in xylem development in Arabidopsis and 64 transgenic plants were produced. CaMsrB2 gene is known to confer drought tolerance in rice and 63 transgenic plants were produced. As a result of PPT leaf painting assay, about 20% of transformation efficiency was observed from 2 times of inoculation. These transgenic plants were confirmed for gene introduction using PCR. Currently, the copy number and the gene expression is investigating using qRT-PCR and RT-PCR. Moreover, 62 lines and 53 lines of T1 seeds from AtRabG3b and CaMsrB2, respectively, were sown in GMO field.

저자
  • Hyun Suk Cho(Dept. of Genetic Engineering, Dong-A University)
  • Hye Jeong Kim(Dept. of Genetic Engineering, Dong-A University)
  • Su Yeong Yun(Dept. of Genetic Engineering, Dong-A University)
  • Yoon Jeong Lee(Dept. of Genetic Engineering, Dong-A University)
  • Hyun Hee Im(Dept. of Genetic Engineering, Dong-A University)
  • Young Soo Chung(Dept. of Genetic Engineering, Dong-A University) Corresponding Author