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Assessment of germination ability in segregated population of colored wheat seeds containing various antioxidant chemicals
Phytochemical in purple percarp of wheat seed consist of high phenolic content including ferulic acid, caffeic acid, vanilic acid and anthocyanins which not only perform as source of distinctive red to purple pigmentation but also high antioxidative material. Previous work has demonstrated that certain pigmentation can be generally regarded as good dietary source of food supplement. Yet, its physiological function in other various aspects has been not thoroughly understood. In this study, we organized fundamental experiment which could evaluate germinating ability of different-colored segregated wheat population. Total of five segregated lines were recognized by assist of CIELAB coordinates. After assessing initial content of total phenol, flavonoid, monomeric anthocyanin and ORAC assay for antioxidant activity from each population, germination assay was taken place in vitro. From germinating grains, sample was taken every 6 hours for measurement of alpha amylase enzyme activity. Discernible difference in chemical constituent was recognized among population along with disparity in ORAC assay. Alpha amylase activity and germination assay showed that darkening of pericarp was related to inhibition of germination. Pigmentation in wheat is important for its physiological role and commodity value which should be considered as critical factor to be integrated in breeding program
