The PHBV nonofibrous membrane fabricated by electron-spinning method for tissue engineered bone regeneration scaffold was evaulated in terms of cellular prolieration and cryopreservation efficiency. The rat calvarial periosteum derived primary cells were cultured with PHBV nanofibrous membranes and analyzed the cellular proliferation and differention fashion and cryopreservation potential by in vitro MTT assay as well as ALP staining and Alizarine red staining with or without cryopreservation for 2 weeks. The rat calvarial periosteum derived primary cells cultured with PHBV nonofibrous membrane showed favorable proliferation and alkaline phosphatase activity with numerous mineral nodule formation regardless of cryopreservation, even though its efficiency was slightly decreased in cryopreserved condition. These findings suggest that PHBV nanofibrous membrane can be applicable as an efficient cell engineered membrane for guided bone regeneration or scaffold for tissue engineered bone regeneration.

Abstract
 Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
 1. 재료
 2. 실험 방법
  가. 세포 채취 및 보관
  나. 세포증식 양상 분석
  다. 골성 분화 정도 평가
 Ⅲ. RESULTS
  가. 세포의 확보 및 저장 효용성
  나. 세포의 증식 양상
  다. 백서두개골막 유래 배양세포의 골성 분화양상
 IV. DISCUSSION
 Ⅵ. REFERENCES

• 신홍인(경북대학교 치의학전문대학원 구강병리학교실) | Hong-In Shin Corresponding author