This study was performed to improve the efficiency of guided bone regeneration by adapting the tissue-engineered bone regeneration approach. The PHBV-collagen nonofibrous membranes cultured with rat calvarial periosteum derived cells in osteogenic culture medium for 7 days were applied to 5 x 2mm size artificial rat tibial defects for 3weeks and evaluated its efficiency as an alternative cell engineered membrane for effective guided bone regeneration by routine histological observation. The defects covered with cell attached PHBV-collagen nanofibrous membrane complexes showed favorable bone repair compared to both membrane non-covered control and membrane only covered group without specific side effects. These findings suggest that the favorable guided bone generation can be achieved by adapting the tissue engineered bone regeneration approach using PHBV-Collagen nanofibrous membrane scaffold.

Abstract
 Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
 1. 실험재료
 2. 실험 방법
  가. 골결손부 형성
  나. PHBV-교원질 나노섬유막 및 PHBV 나노섬유막-세포복합체 이식
  다. 조직학적 관찰
 Ⅲ. RESULTS
 IV. DISCUSSION
 Ⅵ. REFERENCES

• 신홍인(경북대학교 치의학전문대학원 구강병리학교실) | Hong-In Shin Corresponding author