This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with differentaspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphologicalchanges and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular trig-lyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/enhancer-binding protein (C/EBPα), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse tran-scriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts (100μg/mL) of stem and leaf (SALE) and 30%ethanolic extracts (100g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumula-tion during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions (50μg/mL) significantly inhibited lipid dropletaccumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/EBPα and PPARγ) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and effi-cient natural substance to manage obesity.

서 언
 재료 및 방법
  1. 시약 및 재료
  2. 배암차즈기 주정추출물 및 분획물 제조
  3. 3T3-L1 지방전구세포 배양 및 세포 독성 시험
  4. 3T3-L1 지방전구세포의 분화 유도 및 Oil Red O 염색
  5. 3T3-L1 지방세포 내 중성지질 함량 측정
  6. RT-PCR (Reverse-transcription polymerase chain reaction)분석
  7. 통계분석
 
결과 및 고찰
  1. 배암차즈기 조추출물 및 용매분획물의 추출 수율
  2. 지방전구세포 3T3-L1에 대한 세포 독성
  3. 3T3-L1 지방세포 분화 및 지방생성 억제 효과
  4. 3T3-L1 지방세포에서의 중성지방 축적 억제 효과
  5. 지방세포 분화 전사인자 C/EBPα 및 PPARγ mRNA 발현측정
 감사의 글
 REFERENCES

• 최수임(㈜와이디생명과학연구소) | Soo Im Choi Corresponding author
• 곽호영((주)와이디생명과학연구소) | Hoyoung Kwak
• 김재윤(㈜와이디생명과학연구소) | Jae Yoon Kim
• 최종길(㈜와이디생명과학연구소) | Jong Gil Choi
• 이제혁(공주대학교 식품영양학과) | Je Hyuk Lee