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추출조건에 따른 배암차즈기 추출물의 지방세포 분화 및 지방축적 억제 효과 KCI 등재

Antiadipogenic Effects of Salvia plebeia R. Br. Extracts by Extraction Conditions in 3T3-L1 Preadipocytes

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with differentaspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphologicalchanges and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular trig-lyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/enhancer-binding protein (C/EBPα), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse tran-scriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts (100μg/mL) of stem and leaf (SALE) and 30%ethanolic extracts (100g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumula-tion during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions (50μg/mL) significantly inhibited lipid dropletaccumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/EBPα and PPARγ) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and effi-cient natural substance to manage obesity.

목차
서 언
 재료 및 방법
  1. 시약 및 재료
  2. 배암차즈기 주정추출물 및 분획물 제조
  3. 3T3-L1 지방전구세포 배양 및 세포 독성 시험
  4. 3T3-L1 지방전구세포의 분화 유도 및 Oil Red O 염색
  5. 3T3-L1 지방세포 내 중성지질 함량 측정
  6. RT-PCR (Reverse-transcription polymerase chain reaction)분석
  7. 통계분석
 
결과 및 고찰
  1. 배암차즈기 조추출물 및 용매분획물의 추출 수율
  2. 지방전구세포 3T3-L1에 대한 세포 독성
  3. 3T3-L1 지방세포 분화 및 지방생성 억제 효과
  4. 3T3-L1 지방세포에서의 중성지방 축적 억제 효과
  5. 지방세포 분화 전사인자 C/EBPα 및 PPARγ mRNA 발현측정
 감사의 글
 REFERENCES
저자
  • 이제혁(공주대학교 식품영양학과) | Je Hyuk Lee
  • 최종길(㈜와이디생명과학연구소) | Jong Gil Choi
  • 김재윤(㈜와이디생명과학연구소) | Jae Yoon Kim
  • 곽호영((주)와이디생명과학연구소) | Hoyoung Kwak
  • 최수임(㈜와이디생명과학연구소) | Soo Im Choi Corresponding author