In this study, essential oils were extracted from the leaf of Chamaecyparis obtusa (CLEO), indigenous toKorea, CLEO constituents were analysed, and the effects of CLEO on airway hyperresponsiveness (AHR) and airwayinflammation (AI) were investigated in Ovalbumin (OVA)-induced asthma mouse model. Terpenoid components amongidentified CLEO constituents made up more than 80%. The CLEO-treated group in comparison to the control groupshowed reduced AHR, the decrease of eosinophil number in the bronchoalveolar lavage fluid (BALF), reduced specific anti-OVA IgE level in the serum, and a significant reduction in Th2 cytokines levels in the BALF with concentration. We con-cluded that CLEO have an alleviating effect on asthma-like symptoms such as AHR and AI. Further studies about antiasth-matic effect are necessary on the focus of single component of CLEO.

INTRODUCTION
 MATERIALS AND METHODS
  1. Chamaecyparis obtusa leaf essential oil (CLEO)preparation
  2. Analysis of CLEO constitruents.
  3. Animals and breeding condition
  4. OVA-induced asthma mouse model
  5. Classification of experimental groups
  6. Determination of AHR
  7. Collection of blood and BALF
  8. Enzyme-linked immunosorbent assay (ELISA)
  9. Statistical analysis
 RESULTS AND DISCUSSION
  1. Analysis of CLEO constituents
  2. Effect of AHR by CLEO
  3. Effects of inflammatory cells in BALF by CLEO
  4. Effect of a specific anti-OVA IgE in serum by CLEO
  5. Effect of IL-5 and IL-13 levels in BALF by CLEO
 ACKNOWLEDGMENTS
 REFERENCES

• 김성겸(충북대학교 농업생명환경대학 특용식물학과) | Seong Kyeom Kim
• 이승민(충북대학교 농업생명환경대학 특용식물학과) | Seung Min Lee
• 임흥빈(충북대학교 농업생명환경대학 특용식물학과) | Heung Bin Lim Corresponding author