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Analysis of genetic diversity of Codonopsis lanceolata cultivated in Korea using SSR makers

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  • URLhttps://db.koreascholar.com/Article/Detail/302571
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한국육종학회 (The Korean Breeding Society)
초록

Codonopsis lanceolata is a perennial climber. The roots are used as medicinal materials or vegetables. Recently, demand for C. lanceolata is increasing as a healthy food. C. lanceolata is distributed in India and East Asia such as China, Japan as well as Korea. In South Korea, this plant is widely cultivated in Gangwon-do province. No C. lanceolata varieties were developed in Korea. The objective of this study is to analyze genetic diversity of C. lanceolata cultivated in Korea using SSR makers. C. lanceolata roots were collected in each region were cultivated in Chungbuk National University greenhouse. Samples were obtained from fresh leaves of 5 plants from each collection region. The genomic DNA was extracted using CTAB. Genetic diversity was analysed using 4 sets of C. lanceolata SSR makers. PCR was performed in total 20 μL reaction volume containing 20 ng of DNA template, 5 pmole of primers. The genotypes of the analyzed samples were very similar. That means that the genetic diversity of C. lanceolata cultivated in Korea is very low.

저자
  • Serim Kim(Department of Industrial Plant Science & Technology, Chungbuk National University)
  • Ji Hee Jeong(Seed & Seedling Management Division, Korea Forest Seed and Variety Center)
  • Jinsu Gil(Department of Industrial Plant Science & Technology, Chungbuk National University)
  • Tae Dong Kim(Seed & Seedling Management Division, Korea Forest Seed and Variety Center)
  • Yurry Um(National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Ok Tae Kim(National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Ho Bang Kim(Life Sciences Research Institute, Biomedic Co.,Ltd.)
  • Hee Chung(Department of Industrial Plant Science & Technology, Chungbuk National University)
  • Yi Lee(Department of Industrial Plant Science & Technology, Chungbuk National University) Corresponding Author