Zoysiagrass are damaged by fungi diseases such as large patch, dollar spot, pythium blight and brown patch. Large patch is one of the major diseases caused by Rhizoctonia solani AG2-2 on zoysiagrass fields e.g. golf courses. Plant chitinases have been known PR (Pathogen related)-protein. In this study, we isolated two chitinase genes (Zjchi1 and Zjchi2) from zoysiagrass. Antifungal activity analysis revealed that Zjchi2 protein inhibited mycelium extension of fungi. A further study, we cloned 5` upstream region from two chitinase genes for investigating transcription regulatory mechanism that inducing of two chitinase genes dependent R. solani. -818 bp and -799 of upstream region from Zjchi1 and Zjchi2 successfully isolated using in vitro LA (Long and Accurate) PCR system. And then, we generated promoter-GUS reporter constructs with deletion construct based on W-boxes. Constructs were introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation for stable expression of GUS reporter gene.

• Ji-Nam Kang(Faculty of Biotechnology, Jeju National University)
• So-Mi Kang(Faculty of Biotechnology, Jeju National University)
• Hong-Gyu Kang(Subtropical Horticulture Research Institute, Jeju National University)
• Hyeon-Jin Sun(Subtropical Horticulture Research Institute, Jeju National University)
• Yong-Ik Kwon(Subtropical Horticulture Research Institute, Jeju National University)
• Suk-Min Ko(Subtropical Horticulture Research Institute, Jeju National University)
• Hyo-Yeon Lee(Faculty of Biotechnology, Jeju National University, Subtropical Horticulture Research Institute, Jeju National University) Corresponding Author