Next generation sequencing technologies provide opportunities to reveal the genetic variants and differentially expressedgenes. The genetic variants are closely relevance to understanding of genes and phenotypic differences related to agronomic characteristics among cultivars. In this study, we conducted RNA-seq using two Korean soybean accessions, including Daewon and Hwangkeum, by using next generation sequencing against Williams 82 genome as reference. A number of variants such assingle nucleotide variants (SNV), multiple nucleotide variants (MNV), insertion/deletion (InDel) and replacement, was 34,411 and 55,544 in Daewon and Hwangkeum, respectively. Among these variants, 9,611 nonsynonymous variants were detected within 4,290 genes in Daewon and 13,225 non-synonymous variants were located on 5,672 genes in Hwangkeum. The distribution of nonsynonymous variants and expression values of genes can serve as invaluable resource for genotyping and study of traits within genes for soybean improvements.

• Kitae Song(Department of Life Science, Dongguk Univ.-Seoul)
• Jeong Hoon Kim(Department of Life Science, Dongguk Univ.-Seoul)
• Gi Yong Yoon(Department of Life Science, Dongguk Univ.-Seoul)
• Hyo Chul Kim(Department of Life Science, Dongguk Univ.-Seoul)
• Seungho Shin(Department of Life Science, Dongguk Univ.-Seoul)
• Won Cheol Yim(Department of Life Science, Dongguk Univ.-Seoul)
• Kyung-Hee Kim(Department of Life Science, Dongguk Univ.-Seoul)
• Byung-Moo Lee(Department of Life Science, Dongguk Univ.-Seoul) Corresponding author