Insect Growth Regulators (IGRs) are insecticides that disrupt the normal development of target insects by inducing symptoms such as premature molting or supernumerary larval stages. Juvenile hormone systems become the targets of two types of IGRs: the Juvenile Hormone Agonists (JHAs) and Juvenile Hormone Antagonists (JHANs). Pyriproxyfen is one of the chemical compounds widely used as JHA to control many kinds of insects while Kanakugiol is a plant-extracted compound which acts as JHAN.
The small brown planthopper, Laodelphax striatellus, is one of the most serious pest insects of rice plants because it can transmit the rice stripe virus which often causes significant reduction of yield in the field.
In order to analyze the differential gene expressions of L. striatellus upon JHA and JHAN treatment by using next generation sequencing technique, we sprayed Pyriproxyfen and Kanakugiol on 4th instar nymphs of L. striatellus respectively, and extracted total RNA for RNA-seq. The quality-filtered Illumina sequence reads of the control, JHA, and JHAN treated samples were mapped to the reference gene sequences by using the Bowtie2 software. Then the results of mapping by Bowtie2 were analyzed by eXpress software to quantity the differential gene expression.

• Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
• Seok-Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
• Kyu Baik Ha(Department of Agricultural Biotechnology, Seoul National University)
• Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)