Toxicity test of contaminate soil is very complex because of differential bioavailability in the soil. Therefore, bioavailability of metals in soil is a major factor influencing estimates of toxicity. In this study, the two major test was conducted. First, the toxicity of arsenic for the Collembola, Paronychiurus kimi, was assessed by determining the effects of increasing arsenic concentration on survival, reproduction and body concentration of As in five forested soils with different available phosphate and oxide-metal concentration. Second, the sequential extraction procedure (SEP) for arsenic by choosing extraction reagents commonly used for sequential extraction of metals was tested. The EC50 based on total As concentration in soil was estimated respectively. The available phosphate and oxide-metal concentration in soil influenced on As fraction in soil. Especially, As in soil which is non specifically and specifically sorbed (fraction 1, 2) has strong correlation with available phosphate and oxide-metal concentration (p<0.05). The toxicity is more higher in the soil with high available phosphate and low oxide-metal concentration. In addition, the high arsenic concentration in fraction which is amorphous and poor-crystalline hydrous oxide of Fe and Al (fraction 3) had effect to the toxicity. As a result, the toxicity of As is related with As concentration in fraction 1, 2 and 3 and the soil properties and the arsenic fractionation in soil have a influence on the bioavailability and toxicity.