The research was investigated to collect basic data in order to consumption expansion and processed product of Erythronium japonicum. Leaves of Allium japonicum were divided into 17 kinds of amino acid and 38 kinds of free amino acid. Total content of amino acid and free amino acid showed 2,342 mg・100g-1 and 225 mg・100g-1, respectively. Reducing DPPH radical scanvenging half, quantity (IC50 value) of Erythronium japonicum. extraxt 1,000 mg・L-1 were showed the methanol extract (30.8 mg・L-1), ethanol extract (34.1 mg・L-1), hot water extraction water (47.5 mg・L-1). Nitrite scavenging activity in 1,000mg・L-1 of Erythronium japonicum leaf extraxt was followed the ethanol extract (75.0%), methanol extract (74.1%), hot water extraction water (70.3%), respectively. Methannol extract of Erythronium japonicum leaf at 400mg・L-1 inhibited over 99% lung cancer cell (Calu-6), breast cancer cell (NCF-7) and colon cancer cells (HCT-116). Likewise, investigated data will be utilized basic material, and intake of Erythronium japonicum will be enhancing physiological activity, and will be considered to prevent and remedy to lung cancer, breast cancer and colon cancer.