사우디아라비아 북부지역에서 수집된 홍화 총 100자원에 대 한 총폴리페놀 함량 및 항산화 활성을 평가하여 항산화활성이 높은 홍화자원을 선발하고자 연구를 수행하였으며 그 결과는 다음과 같다.
1. 총 100자원의 홍화유전자원에 대한 총폴리페놀 함량은 14.2 ± 0.41 μg GAE mg−1dw에서 81.6 ± 1.56μg GAEmg−1dw 까지 분포하였다. DPPH 라디컬 소거능은 1.6 ± 0.07 μg ASC mg−1 dw에서 14.1 ± 0.23 μg ASCmg−1 dw까지 활성을 나 타냈다. ABTS 활성은 34.5 ± 0.70 μg Trolox mg−1 dw에서 156.5 ± 3.06 μg Trolox mg−1 dw까지 평가되었다. 항산화활성 을 측정한 DPPH와 ABTS 활성간에는 상당한 유의성(r = 0.954**)을 보였다.
2. 홍화자원 100자원의 총폴리페놀 함량 및 항산화활성 결 과값에 대한 상호관계를 분석하여 활성이 높은 그룹(Group1)을 얻었다. Group1에 포함된 8개의 홍화자원은 나머지 그룹에 속한 자원들에 비해 항산화활성이 높았으며, 이중 K185841, K185879, K185863은 Group1내의 다른 자원들보다도 항산화 활성이 상당히 높아 홍화육종이나 기능성물질연구에 좋은 소 재가 될 것으로 사료된다.

This study was undertaken to select genetic resources of safflower with high antioxidant activities. A total of 100 accessions were obtained from the National Agrobiodiversity Center (NAC) of Korea. Total polyphenol contents (TPC) and antioxidant activities of 1,1-diphenyl-2-picryl-hydrazil (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) in 75 % ethanol seed extract of safflower germplasm were determined. TPC ranged from 14.2 ± 0.41 to 81.6 ± 1.56 μg GAE mg-1dry weight (dw). Safflower seed extracts showed variation in DPPH antioxidant activities ranging from 1.6 ± 0.07 to 14.1 ± 0.23 μg ASC mg-1 dw. Antioxidant activities of ABTS ranged from 34.5 ± 0.70 to 156.5 ± 3.06 μg Trolox mg-1 dw. ABTS values showed significant positive correlation (r=0.954**) with DPPH activity. Group I (8 accessions) having high antioxidant activities were obtained by the matrix of calculated distances of TPC and antioxidant activities. K185841, K185879, and K185863 among group I germplasm had higher TPC and antioxidant activities than other accessions. These genotypes are potential sources of natural antioxidants. Therefore, this study provides valuable information for safflower breeders and growers to develop new varieties and produce functional foods.

MATERIALS AND METHODS
Discussion
ACKNOWLEDGMENT
적 요
REFERENCES

• Jung Sook Sung(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 성정숙 Corresponding Author
• Ho Cheol Ko(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 고호철
• On Sook Hur(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 허온숙
• Sang Gyu Kim(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 김상규
• Jung Ro Lee(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 이정로
• Binod P. Luitel(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea)
• Jae Gyun Gwag(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 곽재균
• Hyung Jin Baek(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 백형진
• Kyoung Yul Ryu(National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea) | 류경열 Corresponding Author