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pp.866-870
In this study, coffee waste was extracted with different solvents such as ethyl acetate, methylene chloride and methanol to investigate the total polyphenol contents, electron donating ability and the inhibitory effect on glutathione S-transferase. The total polyphenol contents were 3,060.61±357.12 μg GAE/mL in ethyl acetate, 909.09±35.71 μg GAE/mL in methylene chloride, and 1,602.27±30.36 μg GAE/mL in methanol. The total polyphenol contents showed a significant difference (p<0.05) between the solvents. The electron donating ability was 80.20±1.45% for ethyl acetate, 81.94±0.45% for methylene chloride, and 85.14±1.53% for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The inhibitory effect of the various extracts on glutathione S-transferase (% inhibition) was 92.12±0.56%, 88.48±0245% with methylene chloride extract, and 90.85±0.14% with methanol extract. These too were significant different (p<0.05) between the solvents. The two portions of coffee waste extracts obtained from ethyl acetate and methanol showed meaningful results on the total polyphenol contents, and the inhibition effects on glutathione S-transferase. Therefore, they can be utilized to develop health care foods and can be applied as antioxidants for cosmeceuticals.
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