Mortality of honeybees(Apis cerana) is a serious problem that beekeepers have to face periodically in Korea. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. In this work, we were detected Black queen cell virus (BQCV), Kashmir bee virus (KBV), Korean Sacbrood virus (KSBV), Ascosphaera apis, and Nosema in samples of Apis cerana. Honey bee viruses was detected KSBV(58.5 %), KBV(6.5 %), BQCV(70 %) in 2015 by RT(Revers transcriptase)-PCR. Sacbrood virus (SBV) is an important disease of A. cerana. A. apis can cause chock brood disease to honey bee. It was detected 10.3 % of A. cerana colonies by PCR. Also, Nosema cerana was detected 50.5%. Conclusively, investigated disease of the A. cerana, and confirmed virus that lead to bee disease, this is thought by valuable thing as data for development of beekeeping industry such as Colony Collapse cause searching examination.

• Yong Soo Choi(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)
• Myeong Lyeol Lee(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)
• Man-Young Lee(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)
• Hye-Kyung Kim(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)
• Mi-Young Yoon(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)
• Gyu Ho Byuon(Department of Agricultural Biology, National Academy of Agricultural Science, R.D.A.)