Cotesia plutellae bracovirus (CpBV) is a bracoviral polydnavirus and a parasitic factor of an endoparasitioid wasp, C. plutellae, which parasitizes the diamondback moth (DBM) larvae. CpBV genome was reported to consist of 35 circular segments and encode 157 predicted open reading frames (ORFs). This study revisited and re-annotated the CpBV genome. Total ORFs consist of 15 groups of known gene or gene families as well as hypothetical (HP) group. All 74 HP genes are matched to other PDV genes. RNASeq analysis indicates that 147 genes out of 157 predicted ORFs are expressed in the parasitized DBM at the first day or 7 days after parasitization. At the early parasitic stage, CpBV genes annotated as ELP, BEN, E94K, DUFB, PTP, and HP are highly expressed compared to late parasitic stage. Interestingly, highly expressed genes at the late parasitic stages are HPs.

• Yonggyun Kim(Department of Plant Medicals, College of Natural Sciences, Andong National University)