논문 상세보기
pp.99-109
목적: 새로운 실리콘 모노머를 사용하여 제조한 실리콘하이드로겔 콘택트렌즈와 2종류의 시판용 렌즈에 hyaluronan을 첨가한 다음 lysozyme, albumin, globulin 3종류 단백질에 대한 흡착량의 변화를 살펴보고자 한다. 방법: 실험실에서 제조한 렌즈는 HEMA(2-hydroxyethyl methacrylate, 96%, Junsei)와 TRIM (3-(trimethoxysilyl)propyl methacrylate, 98%, Aldrich) 등의 모노머를 사용하여 cast mould 방법으로 제조하였다. HA(Hyaluronic acid, LifeCore Biomedical) 첨가는 HA 에탄올 용액과 EDC (1-(3-(Dimethylamino)propyl)-3-ethylcarbodiimide methiodide, Aldrich) 용액을 사용하였다. HA의 첨가 전 후 렌즈의 함수율, 광 투과율 등의 물리적 특성 변화와 lysozyme, albumin, globulin이 포함된 인공눈물을 제조하여 48시간 동안 흡착량 변화를 살펴보았다. 각각의 단백질은 HPLC로 정량하였다. 결과: HA가 첨가된 렌즈의 함수율은 첨가 전의 값에 비해 약 10% 증가하였으며, 광 투과율은 큰 변화가 없었다. HA를 첨가한 렌즈에서 시간에 따른 HA의 용출량은 48시간 동안 지수함수 형태로 증가하였다. HA가 첨가되지 않은 렌즈에 비해 HA를 첨가한 모든 렌즈에서 단백질 흡착량이 감소하였으며, 흡착시간이 경과함에 따라 그 감소효과가 크게 줄었다. 단백질의 종류에 따른 흡착량은 lysozyme>globulin>albumin의 순으로 감소하였으며, SHL 렌즈에서 최대 80% 이상 감소하였다. 결 론: Hyaluronan이 첨가된 모든 실리콘하이드로겔 콘택트렌즈에서 단백질 흡착량이 감소하였다. 실리콘하이드로겔 렌즈에 3종류 단백질의 흡착 특성은 렌즈 표면의 친수성에 가장 크게 영향을 받음을 확인할 수 있었으며, 수소결합이 가능한 hyaluronan의 첨가로 인해 렌즈의 친수성을 증가시키고 이로 인해 단백질의 구조적 변성을 방지하여 단백질 흡착을 억제하는 것으로 생각된다.
Purpose: The purpose of this research is to examine the effects of hyaluronan addition on the adsorption properties of three kinds of proteins(lysozyme, albumin, globulin) at the three kinds of silicone hydrogel lenses which one is manufactured by new silicone monomer in the laboratory and the other two are commercial silicone hydrogel lenses. Methods: The contact lens was fabricated by HEMA(2-hydroxyethyl methacrylate, 96%, Junsei) and TRIM(3-(trimethoxysilyl)propyl methacrylate, 98%, Aldrich) using the cast mold method. To add the HA(Hyaluronic acid, LifeCore Biomedical) to the lens, each lens was placed in the HA ethanol solution and EDC(1-(3-(Dimethylamino) propyl)-3-ethyl carbodiimide methiodide, Aldrich) solution for 24h. Physical properties such as water contents and optical transmittance on the HA-added lens were examined. To measure the variation of the amounts of protein adsorbed on the each lenses for 48 hours, we prepared the artificial tears containing lysozyme, albumin, globulin and used it for each protein adsorption. HPLC was used to analyze the amount of the protein adsorption. Result: The water content of the contact lens added with HA increased about 10% compared to the silicone contact lens used as the control, and no big changes was observed in the light transmittance. The amount of HA released from the HA-added lens was increased as an exponentially for 48 hours. The amount of protein adsorption was decreased at all HA-added lenses, and the reduction rate was greatly decreased with adsorption time. The amount of protein adsorption was decreased in order of lysozyme>globulin>albumin, and maximum decrease in the protein adsorption was observed at the SHL lens which was more than 80% reduction. Conclusion: The amount of protein adsorption was decreased at all silicone hydrogel contact lens added with HA. It was confirmed that the adsorption property of the three kinds of the protein on the silicone hydrogel contact lens was greatly affected by the hydrophilic of the lens surface. Addition of hyaluronan which is able to produce hydrogen bonding increases the hydrophilic property of the lens, and prevents the structural denaturalization of the proteins, resulting in reduction of protein adsorption.
