We isolated 105 strains of lactic acid bacteria (LAB) from home-made kimchi and breast milk-fed Korean infant feces on the basis of morphological analysis. This study aimed to compare the probiotic characteristics of selected strains of LAB including bile and acid tolerances, cholesterol assimilation and adhesion activity. Among the isolates of LAB, 54 isolates were identified as Lactobacillus plantarum (14 strains), L. brevis (12 strains), L. sakei (9 strains), L. acidophilus (3 strains), L. casei (1 strain), and L. fermentum (1 strain). Acid tolerances under artificial gastric juice, pH 2.5 for 2 h at 37°C, were significantly different among the Lactobacillus species. Lactobacillus acidophilus and L. plantarum strains exhibited high tolerance in acid and bile. Lactobacillus acidophilus strains exhibited high cholesterol assimilation activity and showed a significantly higher tolerance to 0.3% bile acid than other Lactobacillus strains (p<0.05). Based on these results, we selected the best strain, named NS1 (L. acidophilus) as a potential probiotics that can be utilized in the manufacturing of dairy foods and dietary supplements.

서 론
 재료 및 방법
  유산균의 분리
  내담즙산성 평가
  내산성 평가
  콜레스테롤 저하 작용
  장상피세포 부착능력
  유산균의 16S rDNA 추출
  유산균의 동정
 결과 및 고찰
  내담즙산성 및 내산성 조사
  콜레스테롤 저하작용 및 장상피 세포 부착능력
  선발 유산균의 분석
 요 약
 참고문헌

• 신미영(전남대학교 동물자원학부) | Miyoung Shin
• 송수연(전남대학교 동물자원학부) | Sooyeon Song
• 문용일(우석대학교 동물자원식품학과) | Yong-Il Moon
• 오세종(전남대학교 동물자원학부) | Sejong Oh Corresponding author