The aim of this study was to compare ultrasonic measured traits (Eye muscle area : EMA, backfat thickness : BF and marbling score : MS) of Hanwoo cows by months of age and periods around 1st calving. The data used in this study were taken 2,636 cows which were measured from 20 months old to 28 months old and within 5 months around 1st calving. According to comparison of cows' month of age, ultrasonic measured EMA, BF and MS were not significantly different between 21 months old and 26 months old, 21 months old and 25 months old and 23 months old and 27 months old, respectively. According to comparison of periods around 1st calving, ultrasonic measured EMA, BF and MS were not significantly different between -45 days and 30 days, -15 days and 75 days, -105 days and 90 days around 1st calving, respectively. The selection of cows could be possible based on the phenotypic ultrasonic measurement performance if the traits are measured between 23-month old and 25-month old and between - 15 days and 30 days around 1st calving.