This study was carried out to investigate and assess the cold tolerance of 24 species broad-leaved evergreen trees in southern region, South Korea, and propose the selection for urban greening responsive to the climate change. The cold stressed impact of each species was measured and calculated by the electrolyte leakage (EL) method, and then the lethal temperature was predicted by the non-linear regression analysis. The scattered plots and fitted curves of most species tended to show sigmoidal response curve. On assessing the EL values and sigmoidal response curve pattern with different temperature, the differences were obviously showed among all the species. Also, among the species within the same family, the differences were obviously showed. The maximum temperature difference among the species was over 10℃. Between Ilex rutunda and Ilex integra within the same family, Aquifoliaceae, it was over 10℃. The results indicate that there are significant differences in cold tolerance among different species in the same region, which are not affected by any environmental factors but affected by any genetical factors. Thus it is valuable to assess the cold tolerance on most broad-leaved evergreen trees in southern region, South Korea. As a result, Euonymus japonicus, Trachelospermum asiaticum, Dendropanax morbiferus, Ilex integra, Machilus thunbergii, Ilex x wandoensis, Cinnamomum japonicum, Distylium racemosum, and Castanopsis sieboldii may have better cold tolerance and survive the region closer to middle region, South Korea compared to the others.